mSphere (Oct 2021)

Viability-Resolved Metagenomics Reveals Antagonistic Colonization Dynamics of Staphylococcus epidermidis Strains on Preterm Infant Skin

  • K. Taylor Hellmann,
  • Carly E. Tuura,
  • James Fish,
  • Jaimin M. Patel,
  • D. Ashley Robinson

DOI
https://doi.org/10.1128/mSphere.00538-21
Journal volume & issue
Vol. 6, no. 5

Abstract

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The skin is a habitat for microbes that commonly infect preterm infants, but the use of sequencing for fine-scale study of the microbial communities of skin that develop in these infants has been limited by technical barriers. We treated skin swabs of preterm infants with a photoreactive dye that eliminates DNA from nonviable microbes and then sequenced the remaining DNA.