Cancer Management and Research (Jul 2021)
KLF7 Promotes Gastric Carcinogenesis Through Regulation of ANTXR1
Abstract
Yuanchun Li,1,* Qingdong Wang,2,* DongWei Wang,2 Weihua Fu3 1Department of General Surgery, The Second Affiliated Hospital of Qiqihar Medical University, Qiqihar, Heilongjiang Province, People’s Republic of China; 2Department of Anesthesiology, First Affiliated Hospital of Jiamusi University, Jiamusi, Heilongjiang, People’s Republic of China; 3Department of General Surgery, Tianjin Medical University General Hospital, Tianjin, People’s Republic of China*These authors contributed equally to this workCorrespondence: DongWei WangDepartment of Anesthesiology, First Affiliated Hospital of Jiamusi University, No. 348, Dexiang Street, Jiamusi, Heilongjiang Province, 154002, People’s Republic of ChinaTel +86-0454-8605850Email [email protected] FuDepartment of General Surgery, Tianjin Medical University General Hospital, No. 154, Anshan Road, Tianjin, 300052, People’s Republic of ChinaTel +022-60363901Email [email protected]: Elucidating the mechanism of gastric cancer progression is of great importance for the discovery of new therapy targets against gastric cancer. In this study, we investigated the function of Kruppel-like factor 7 (KLF7) in gastric cancer.Methods: qPCR and Western blot were performed to determine the expression of ANTXR1 after KLF7 inhibition. CCK-8, colony formation, apoptosis analysis, cell cycle analysis and transwell assay were performed to determine KLF7 functions in cellular proliferation, migration, apoptosis and cell cycle. Tumour xenograft experiments were performed to examine cell growth in vivo.Results: The results showed that KLF7 was upregulated in gastric cancer. The proliferation and migration of gastric cancer cells were suppressed by depletion of KLF7. In vivo tumour progression was also attenuated following the downregulation of KLF7. Meanwhile, overexpression of KLF7 promoted the proliferation and migration of gastric cancer cells. The results of the mechanistic analysis showed that KLF7 promoted gastric carcinogenesis via upregulation of ANTXR cell adhesion molecule 1 (ANTXR1).Conclusion: Therefore, this study may provide a theoretical foundation for further clinical therapy of gastric cancer.Keywords: KLF7, gastric cancer, ANTXR1, proliferation, migration
