Molecules (Jul 2020)

Combined MS/MS-NMR Annotation Guided Discovery of <i>Iris lactea</i> var. <i>chinensis</i> Seed as a Source of Viral Neuraminidase Inhibitory Polyphenols

  • Hyun Woo Kim,
  • Soo Sung Kim,
  • Kyo Bin Kang,
  • Byeol Ryu,
  • Eunjin Park,
  • Jungmoo Huh,
  • Won Kyung Jeon,
  • Hee-Sung Chae,
  • Won Keun Oh,
  • Jinwoong Kim,
  • Sang Hyun Sung,
  • Young-Won Chin

DOI
https://doi.org/10.3390/molecules25153383
Journal volume & issue
Vol. 25, no. 15
p. 3383

Abstract

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In this study, the chemical diversity of polyphenols in Iris lactea var. chinensis seeds was identified by combined MS/MS-NMR analysis. Based on the annotated chemical profile, the isolation of stilbene oligomers was conducted, and consequently, stilbene oligomers (1-10) were characterized. Of these, compounds 1 and 2 are previously undescribed stilbene dimer glycoside (1) and tetramer glycoside (2), respectively. Besides, to evaluate this plant seed as a rich source of stilbene oligomers, we quantified three stilbene oligomers of I. lactea var. chinensis seeds. The contents of three major stilbene oligomers—trans-ε-viniferin (3), vitisin A (6), and vitisin B (9)—in I. lactea var. chinensis seeds were quantified as 2.32 (3), 4.95 (6), and 1.64 (9) mg/g dry weight (DW). All the isolated compounds were tested for their inhibitory activities against influenza neuraminidase. Compound 10 was found to be active with the half maximal inhibitory concentration (IC50) values at 4.76 μM. Taken together, it is concluded that I. lactea var. chinensis seed is a valuable source of stilbene oligomers with a human health benefit.

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