Simultaneous Analysis of MLH1, MSH2, MSH6, PMS2 and KRAS in Patients with Gastric and Colon Cancer Using Stochastic Sensors

Abstract

Two stochastic sensors were characterized and validated for the molecular identification and quantification of MLH1, MSH2, MSH6, PMS2 and KRAS in biological samples using two types of doped-graphene modified with maltodextrin. When a potential of 125 mV vs. Ag/AgCl was applied, the two stochastic sensors recorded extremely low limits of determination (fg mL−1 magnitude order) and wide linear concentration ranges, which enabled the molecular identification and quantification of MLH1, MSH2, MSH6, PMS2 and KRAS in healthy individuals and patients with gastric or colon cancer. The recoveries of MLH1, MSH2, MSH6, PMS2 and KRAS in whole blood, saliva, urine and tumoral tissue samples exceeded 98.00% with a relative standard deviation of less than 1.00%.

Keywords

• gastric cancer
• colon cancer
• stochastic sensor
• MLH1
• MSH2
• MSH6