mBio (Feb 2021)
Quantifying Absolute Neutralization Titers against SARS-CoV-2 by a Standardized Virus Neutralization Assay Allows for Cross-Cohort Comparisons of COVID-19 Sera
- Kasopefoluwa Y. Oguntuyo,
- Christian S. Stevens,
- Chuan Tien Hung,
- Satoshi Ikegame,
- Joshua A. Acklin,
- Shreyas S. Kowdle,
- Jillian C. Carmichael,
- Hsin-Ping Chiu,
- Kristopher D. Azarm,
- Griffin D. Haas,
- Fatima Amanat,
- Jéromine Klingler,
- Ian Baine,
- Suzanne Arinsburg,
- Juan C. Bandres,
- Mohammed N. A. Siddiquey,
- Robert M. Schilke,
- Matthew D. Woolard,
- Hongbo Zhang,
- Andrew J. Duty,
- Thomas A. Kraus,
- Thomas M. Moran,
- Domenico Tortorella,
- Jean K. Lim,
- Andrea V. Gamarnik,
- Catarina E. Hioe,
- Susan Zolla-Pazner,
- Stanimir S. Ivanov,
- Jeremy P. Kamil,
- Florian Krammer,
- Benhur Lee
Affiliations
- Kasopefoluwa Y. Oguntuyo
- ORCiD
- Department of Microbiology, Icahn School of Medicine at Mount Sinai, New York, New York, USA
- Christian S. Stevens
- ORCiD
- Department of Microbiology, Icahn School of Medicine at Mount Sinai, New York, New York, USA
- Chuan Tien Hung
- ORCiD
- Department of Microbiology, Icahn School of Medicine at Mount Sinai, New York, New York, USA
- Satoshi Ikegame
- Department of Microbiology, Icahn School of Medicine at Mount Sinai, New York, New York, USA
- Joshua A. Acklin
- Department of Microbiology, Icahn School of Medicine at Mount Sinai, New York, New York, USA
- Shreyas S. Kowdle
- Department of Microbiology, Icahn School of Medicine at Mount Sinai, New York, New York, USA
- Jillian C. Carmichael
- ORCiD
- Department of Microbiology, Icahn School of Medicine at Mount Sinai, New York, New York, USA
- Hsin-Ping Chiu
- Department of Microbiology, Icahn School of Medicine at Mount Sinai, New York, New York, USA
- Kristopher D. Azarm
- Department of Microbiology, Icahn School of Medicine at Mount Sinai, New York, New York, USA
- Griffin D. Haas
- Department of Microbiology, Icahn School of Medicine at Mount Sinai, New York, New York, USA
- Fatima Amanat
- Department of Microbiology, Icahn School of Medicine at Mount Sinai, New York, New York, USA
- Jéromine Klingler
- Division of Infectious Diseases, Department of Medicine, Icahn School of Medicine at Mount Sinai, New York, New York, USA
- Ian Baine
- Department of Pathology, Molecular, and Cell-Based Medicine, Icahn School of Medicine at Mount Sinai, New York, New York, USA
- Suzanne Arinsburg
- Department of Pathology, Molecular, and Cell-Based Medicine, Icahn School of Medicine at Mount Sinai, New York, New York, USA
- Juan C. Bandres
- Division of Infectious Diseases, Department of Medicine, Icahn School of Medicine at Mount Sinai, New York, New York, USA
- Mohammed N. A. Siddiquey
- Department of Microbiology and Immunology, Louisiana State University Health Science Center Shreveport, Shreveport, Louisiana, USA
- Robert M. Schilke
- Department of Microbiology and Immunology, Louisiana State University Health Science Center Shreveport, Shreveport, Louisiana, USA
- Matthew D. Woolard
- Department of Microbiology and Immunology, Louisiana State University Health Science Center Shreveport, Shreveport, Louisiana, USA
- Hongbo Zhang
- Department of Microbiology and Immunology, Louisiana State University Health Science Center Shreveport, Shreveport, Louisiana, USA
- Andrew J. Duty
- Department of Microbiology, Icahn School of Medicine at Mount Sinai, New York, New York, USA
- Thomas A. Kraus
- Department of Microbiology, Icahn School of Medicine at Mount Sinai, New York, New York, USA
- Thomas M. Moran
- Department of Microbiology, Icahn School of Medicine at Mount Sinai, New York, New York, USA
- Domenico Tortorella
- Department of Microbiology, Icahn School of Medicine at Mount Sinai, New York, New York, USA
- Jean K. Lim
- Department of Microbiology, Icahn School of Medicine at Mount Sinai, New York, New York, USA
- Andrea V. Gamarnik
- Department of Microbiology, Icahn School of Medicine at Mount Sinai, New York, New York, USA
- Catarina E. Hioe
- Department of Microbiology, Icahn School of Medicine at Mount Sinai, New York, New York, USA
- Susan Zolla-Pazner
- ORCiD
- Department of Microbiology, Icahn School of Medicine at Mount Sinai, New York, New York, USA
- Stanimir S. Ivanov
- Department of Microbiology and Immunology, Louisiana State University Health Science Center Shreveport, Shreveport, Louisiana, USA
- Jeremy P. Kamil
- ORCiD
- Department of Microbiology and Immunology, Louisiana State University Health Science Center Shreveport, Shreveport, Louisiana, USA
- Florian Krammer
- ORCiD
- Department of Microbiology, Icahn School of Medicine at Mount Sinai, New York, New York, USA
- Benhur Lee
- ORCiD
- Department of Microbiology, Icahn School of Medicine at Mount Sinai, New York, New York, USA
- DOI
- https://doi.org/10.1128/mBio.02492-20
- Journal volume & issue
-
Vol. 12,
no. 1
Abstract
Vaccines and antibody-based therapeutics like convalescent-phase plasma therapy are premised upon inducing or transferring neutralizing antibodies that inhibit SARS-CoV-2 entry into cells. Virus neutralization assays (VNAs) for measuring neutralizing antibody titers (NATs) are an essential part of determining vaccine or therapeutic efficacy.
