Frontiers in Plant Science (Jan 2023)
Gibberellins as a novel mutagen for inducing 2n gametes in plants
- Yifan Zhao,
- Yifan Zhao,
- Yifan Zhao,
- Bo Kong,
- Bo Kong,
- Bo Kong,
- Phuong Uyen Do,
- Phuong Uyen Do,
- Phuong Uyen Do,
- Liang Li,
- Liang Li,
- Liang Li,
- Jiahua Du,
- Jiahua Du,
- Jiahua Du,
- Lexun Ma,
- Lexun Ma,
- Lexun Ma,
- Yaru Sang,
- Yaru Sang,
- Yaru Sang,
- Jian Wu,
- Jian Wu,
- Jian Wu,
- Qing Zhou,
- Qing Zhou,
- Qing Zhou,
- Xuetong Cheng,
- Xuetong Cheng,
- Xuetong Cheng,
- Xiangyang Kang,
- Xiangyang Kang,
- Xiangyang Kang,
- Pingdong Zhang,
- Pingdong Zhang,
- Pingdong Zhang
Affiliations
- Yifan Zhao
- National Engineering Research Center of Tree Breeding and Ecological Restoration, Beijing Forestry University, Beijing, China
- Yifan Zhao
- Key Laboratory of Genetics and Breeding in Forest Trees and Ornamental Plants, Ministry of Education, Beijing Forestry University, Beijing, China
- Yifan Zhao
- College of Biological Sciences and Technology, Beijing Forestry University, Beijing, China
- Bo Kong
- National Engineering Research Center of Tree Breeding and Ecological Restoration, Beijing Forestry University, Beijing, China
- Bo Kong
- Key Laboratory of Genetics and Breeding in Forest Trees and Ornamental Plants, Ministry of Education, Beijing Forestry University, Beijing, China
- Bo Kong
- College of Biological Sciences and Technology, Beijing Forestry University, Beijing, China
- Phuong Uyen Do
- National Engineering Research Center of Tree Breeding and Ecological Restoration, Beijing Forestry University, Beijing, China
- Phuong Uyen Do
- Key Laboratory of Genetics and Breeding in Forest Trees and Ornamental Plants, Ministry of Education, Beijing Forestry University, Beijing, China
- Phuong Uyen Do
- College of Biological Sciences and Technology, Beijing Forestry University, Beijing, China
- Liang Li
- National Engineering Research Center of Tree Breeding and Ecological Restoration, Beijing Forestry University, Beijing, China
- Liang Li
- Key Laboratory of Genetics and Breeding in Forest Trees and Ornamental Plants, Ministry of Education, Beijing Forestry University, Beijing, China
- Liang Li
- College of Biological Sciences and Technology, Beijing Forestry University, Beijing, China
- Jiahua Du
- National Engineering Research Center of Tree Breeding and Ecological Restoration, Beijing Forestry University, Beijing, China
- Jiahua Du
- Key Laboratory of Genetics and Breeding in Forest Trees and Ornamental Plants, Ministry of Education, Beijing Forestry University, Beijing, China
- Jiahua Du
- College of Biological Sciences and Technology, Beijing Forestry University, Beijing, China
- Lexun Ma
- National Engineering Research Center of Tree Breeding and Ecological Restoration, Beijing Forestry University, Beijing, China
- Lexun Ma
- Key Laboratory of Genetics and Breeding in Forest Trees and Ornamental Plants, Ministry of Education, Beijing Forestry University, Beijing, China
- Lexun Ma
- College of Biological Sciences and Technology, Beijing Forestry University, Beijing, China
- Yaru Sang
- National Engineering Research Center of Tree Breeding and Ecological Restoration, Beijing Forestry University, Beijing, China
- Yaru Sang
- Key Laboratory of Genetics and Breeding in Forest Trees and Ornamental Plants, Ministry of Education, Beijing Forestry University, Beijing, China
- Yaru Sang
- College of Biological Sciences and Technology, Beijing Forestry University, Beijing, China
- Jian Wu
- National Engineering Research Center of Tree Breeding and Ecological Restoration, Beijing Forestry University, Beijing, China
- Jian Wu
- Key Laboratory of Genetics and Breeding in Forest Trees and Ornamental Plants, Ministry of Education, Beijing Forestry University, Beijing, China
- Jian Wu
- College of Biological Sciences and Technology, Beijing Forestry University, Beijing, China
- Qing Zhou
- National Engineering Research Center of Tree Breeding and Ecological Restoration, Beijing Forestry University, Beijing, China
- Qing Zhou
- Key Laboratory of Genetics and Breeding in Forest Trees and Ornamental Plants, Ministry of Education, Beijing Forestry University, Beijing, China
- Qing Zhou
- College of Biological Sciences and Technology, Beijing Forestry University, Beijing, China
- Xuetong Cheng
- National Engineering Research Center of Tree Breeding and Ecological Restoration, Beijing Forestry University, Beijing, China
- Xuetong Cheng
- Key Laboratory of Genetics and Breeding in Forest Trees and Ornamental Plants, Ministry of Education, Beijing Forestry University, Beijing, China
- Xuetong Cheng
- College of Biological Sciences and Technology, Beijing Forestry University, Beijing, China
- Xiangyang Kang
- National Engineering Research Center of Tree Breeding and Ecological Restoration, Beijing Forestry University, Beijing, China
- Xiangyang Kang
- Key Laboratory of Genetics and Breeding in Forest Trees and Ornamental Plants, Ministry of Education, Beijing Forestry University, Beijing, China
- Xiangyang Kang
- College of Biological Sciences and Technology, Beijing Forestry University, Beijing, China
- Pingdong Zhang
- National Engineering Research Center of Tree Breeding and Ecological Restoration, Beijing Forestry University, Beijing, China
- Pingdong Zhang
- Key Laboratory of Genetics and Breeding in Forest Trees and Ornamental Plants, Ministry of Education, Beijing Forestry University, Beijing, China
- Pingdong Zhang
- College of Biological Sciences and Technology, Beijing Forestry University, Beijing, China
- DOI
- https://doi.org/10.3389/fpls.2022.1110027
- Journal volume & issue
-
Vol. 13
Abstract
The plant hormone gibberellin (GA) regulates many physiological processes, such as cell differentiation, cell elongation, seed germination, and the response to abiotic stress. Here, we found that injecting male flower buds with exogenous gibberellic acid (GA3) caused defects in meiotic cytokinesis by interfering with radial microtubule array formation resulting in meiotic restitution and 2n pollen production in Populus. A protocol for inducing 2n pollen in Populus with GA3 was established by investigating the effects of the dominant meiotic stage, GA3 concentration, and injection time. The dominant meiotic stage (F = 41.882, P < 0.001) and GA3 injection time (F = 172.466, P < 0.001) had significant effects on the frequency of induced 2n pollen. However, the GA3 concentration (F = 1.391, P = 0.253) did not have a significant effect on the frequency of induced 2n pollen. The highest frequency of GA3-induced 2n pollen (21.37%) was observed when the dominant meiotic stage of the pollen mother cells was prophase II and seven injections of 10 μM GA3 were given. Eighteen triploids were generated from GA3-induced 2n pollen. Thus, GA3 can be exploited as a novel mutagen to induce flowering plants to generate diploid male gametes. Our findings provide some new insight into the function of GAs in plants.
Keywords
