Frontiers in Plant Science (Nov 2021)
OVATE Family Protein PpOFP1 Physically Interacts With PpZFHD1 and Confers Salt Tolerance to Tomato and Yeast
- Qiuping Tan,
- Qiuping Tan,
- Qiuping Tan,
- Qiuping Tan,
- Shan Jiang,
- Ning Wang,
- Ning Wang,
- Ning Wang,
- Xiao Liu,
- Xiao Liu,
- Xiao Liu,
- Xinhao Zhang,
- Xinhao Zhang,
- Xinhao Zhang,
- Binbin Wen,
- Binbin Wen,
- Binbin Wen,
- Yuhui Fang,
- Yuhui Fang,
- Yuhui Fang,
- Huajie He,
- Huajie He,
- Huajie He,
- Xiude Chen,
- Xiude Chen,
- Xiude Chen,
- Xiling Fu,
- Xiling Fu,
- Xiling Fu,
- Dongmei Li,
- Dongmei Li,
- Dongmei Li,
- Wei Xiao,
- Wei Xiao,
- Wei Xiao,
- Ling Li,
- Ling Li,
- Ling Li
Affiliations
- Qiuping Tan
- College of Horticulture Science and Engineering, Shandong Agricultural University, Tai’an, China
- Qiuping Tan
- State Key Laboratory of Crop Biology, Shandong Agricultural University, Tai’an, China
- Qiuping Tan
- Shandong Collaborative Innovation Center for Fruit and Vegetable Production With High Quality and Efficiency, Tai’an, China
- Qiuping Tan
- College of Life Sciences, Shandong Agricultural University, Tai’an, China
- Shan Jiang
- Shandong Huayu University of Technology, Dezhou, China
- Ning Wang
- College of Horticulture Science and Engineering, Shandong Agricultural University, Tai’an, China
- Ning Wang
- State Key Laboratory of Crop Biology, Shandong Agricultural University, Tai’an, China
- Ning Wang
- Shandong Collaborative Innovation Center for Fruit and Vegetable Production With High Quality and Efficiency, Tai’an, China
- Xiao Liu
- College of Horticulture Science and Engineering, Shandong Agricultural University, Tai’an, China
- Xiao Liu
- State Key Laboratory of Crop Biology, Shandong Agricultural University, Tai’an, China
- Xiao Liu
- Shandong Collaborative Innovation Center for Fruit and Vegetable Production With High Quality and Efficiency, Tai’an, China
- Xinhao Zhang
- College of Horticulture Science and Engineering, Shandong Agricultural University, Tai’an, China
- Xinhao Zhang
- State Key Laboratory of Crop Biology, Shandong Agricultural University, Tai’an, China
- Xinhao Zhang
- Shandong Collaborative Innovation Center for Fruit and Vegetable Production With High Quality and Efficiency, Tai’an, China
- Binbin Wen
- College of Horticulture Science and Engineering, Shandong Agricultural University, Tai’an, China
- Binbin Wen
- State Key Laboratory of Crop Biology, Shandong Agricultural University, Tai’an, China
- Binbin Wen
- Shandong Collaborative Innovation Center for Fruit and Vegetable Production With High Quality and Efficiency, Tai’an, China
- Yuhui Fang
- College of Horticulture Science and Engineering, Shandong Agricultural University, Tai’an, China
- Yuhui Fang
- State Key Laboratory of Crop Biology, Shandong Agricultural University, Tai’an, China
- Yuhui Fang
- Shandong Collaborative Innovation Center for Fruit and Vegetable Production With High Quality and Efficiency, Tai’an, China
- Huajie He
- College of Horticulture Science and Engineering, Shandong Agricultural University, Tai’an, China
- Huajie He
- State Key Laboratory of Crop Biology, Shandong Agricultural University, Tai’an, China
- Huajie He
- Shandong Collaborative Innovation Center for Fruit and Vegetable Production With High Quality and Efficiency, Tai’an, China
- Xiude Chen
- College of Horticulture Science and Engineering, Shandong Agricultural University, Tai’an, China
- Xiude Chen
- State Key Laboratory of Crop Biology, Shandong Agricultural University, Tai’an, China
- Xiude Chen
- Shandong Collaborative Innovation Center for Fruit and Vegetable Production With High Quality and Efficiency, Tai’an, China
- Xiling Fu
- College of Horticulture Science and Engineering, Shandong Agricultural University, Tai’an, China
- Xiling Fu
- State Key Laboratory of Crop Biology, Shandong Agricultural University, Tai’an, China
- Xiling Fu
- Shandong Collaborative Innovation Center for Fruit and Vegetable Production With High Quality and Efficiency, Tai’an, China
- Dongmei Li
- College of Horticulture Science and Engineering, Shandong Agricultural University, Tai’an, China
- Dongmei Li
- State Key Laboratory of Crop Biology, Shandong Agricultural University, Tai’an, China
- Dongmei Li
- Shandong Collaborative Innovation Center for Fruit and Vegetable Production With High Quality and Efficiency, Tai’an, China
- Wei Xiao
- College of Horticulture Science and Engineering, Shandong Agricultural University, Tai’an, China
- Wei Xiao
- State Key Laboratory of Crop Biology, Shandong Agricultural University, Tai’an, China
- Wei Xiao
- Shandong Collaborative Innovation Center for Fruit and Vegetable Production With High Quality and Efficiency, Tai’an, China
- Ling Li
- College of Horticulture Science and Engineering, Shandong Agricultural University, Tai’an, China
- Ling Li
- State Key Laboratory of Crop Biology, Shandong Agricultural University, Tai’an, China
- Ling Li
- Shandong Collaborative Innovation Center for Fruit and Vegetable Production With High Quality and Efficiency, Tai’an, China
- DOI
- https://doi.org/10.3389/fpls.2021.759955
- Journal volume & issue
-
Vol. 12
Abstract
The OVATE family protein (OFP) genes (OFPs) have been shown to respond to salt stress in plants. However, the regulatory mechanism for salt tolerance of the peach (Prunus persica) OFP gene PpOFP1 has not been elucidated. In this study, using yeast two-hybrid screening, we isolated a nucleus-localized ZF-HD_dimer domain protein PpZFHD1, which interacts with the PpOFP1 protein in the peach cultivar “Zhongnongpan No.10”. A segmentation experiment further suggested that the interaction happens more specifically between the N-terminal, contains ZF-HD_dimer domain, of PpZFHD1 and the C-terminal, consists of OVATE domain, of PpOFP1. Additionally, quantitative real-time polymerase chain reaction (qRT-PCR) experiments indicate that transcription of these two genes are induced by 200 mmol/L (mM) NaCl treatment. Heterogeneous transformation experiments suggested that the growth status of transformed yeast strain over-expressing each of these two genes was more robust than that of control (CK). Furthermore, transgenic tomato plants over-expressing PpOFP1 were also more robust. They had a higher content of chlorophyll, soluble proteins, soluble sugars, and proline. Activities of the superoxide dismutase (SOD), peroxidase (POD), and catalase (CAT) in these plants were higher, and tissues from these plants exhibited a lower relative conductivity and malondialdehyde (MDA) content. These results suggest that PpOFP1 physically interacts with PpZFHD1 and confers salt tolerance to tomato and yeast, thus revealing a novel mechanism for regulating salt tolerance in peach and other perennial deciduous trees.
Keywords
- Prunus persica
- salt tolerance
- OVATE Family Protein PpOFP1
- ZF-HD_dimer domain protein PpZFHD1
- protein interaction
