Journal of Lipid Research (Oct 1959)

Quantitative glass paper chromatography: phosphatidyl choline and sphingomyelin

  • James E. Muldrey,
  • O. Neal Miller,
  • James G. Hamilton

Journal volume & issue
Vol. 1, no. 1
pp. 48 – 52

Abstract

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A rapid chromatographic procedure was developed for the separation of sphingomyelin, phosphatidyl choline, phosphatidyl ethanolamine, phosphatidyl serine, phosphatidyl inositol, and free fatty acids on glass paper coated with sodium silicate. In addition, phosphatidyl choline and sphingomyelin were determined quantitatively by densitometry of the charred chromatogram, which was obtained by spraying the developed chromatogram with sulfuric acid and heating in an oven. The separation of phosphatides on sodium silicate-treated glass paper is more rapid than on silicic acid-impregnated paper, and the former is simpler to prepare. Preliminary application of this quantitative technique to human serum indicates that it may have a wide adaptability for the determination of phospholipids in natural products.