Enhancement of bioethanol production from lignocellulosic biomass of banana by single batch fermentation using Saccharomyces cerevisiae and native microorganism

Abstract

Bioethanol production using the lignocellulosic biomass of banana viz., banana raw peel, ripe peel and pseudostem were attempted. Among the three feed stocks, maximum total reducing sugar content of 21.98% was observed in the banana ripe peel. Pretreatments of the feed stocks with acid resulted in higher lignin removal and increased total reducing sugar content compared to the alkali treatment. Separate Hydrolysis and Fermentation (SHF), Simultaneous Saccharification and Fermentation (SSF) and Single Batch Bioconversion (SBB) were carried out for the fermentation process using Saccharomyces cerevisiae. By SSF fermentation processs, 6.63% of ethanol was produced by Saccharomyces cerevisiae from the untreated samples of banana raw peel. Enhancement of bioethanol production was done using a native cellulolytic microorganism isolated from the degraded banana samples. Using the native microorganism along with Saccharomyces cerevisiae in SBB resulted in 6.88% of bioethanol conversion. This is the first report of using native microorganism for enhanced degradation of cellulose in banana biomass for higher bioethanol production.

Keywords

• Banana raw peel
• Bioethanol
• Native microorganism
• Saccharomyces cerevisiae
• Single batch bioconversion