Biosynthesis of silver nano particles (AgNPs) from blue green algae (Arthrospira platensis) and their anti-pathogenic applications

Abstract

The increase in bacterial infections and the emergence of antibiotic resistance has led to the development of bio-derived and environmentally friendly nanomaterials to counteract bacterial activity, and silver nanoparticles AgNPs are a promising way to achieve this. The present work aimed to synthesize AgNPs from the blue-green algae Athrospira Platensis and evaluate their antibacterial efficacy against Enterococcus faecalis, Staphylococcus aureus, Proteus mirabilis, and Pseudomonas aeruginosa isolated from human urinary tract infections. The results were shown and confirmed by peak UV spectra at 450. Through SEM and DLS analyses, the shapes of the AgNPs were determined to be spherical and with sizes ranging from 15 to 100 nm. The zeta potential values of AgNPs were found to be −20 ± 3.2 mV indicating the absence of AgNPs aggregates. Synthetic AgNPs produced by A. platensis showed a strong antibacterial effect against bacteria isolated from human urinary tract infections. The diameters of the inhibitory zone for S. aureus E. faecalis P. mirabilis and P. aeruginosa 14 mm, 16 mm, 15 mm, and 12 mm, respectively. AgNPs showed the highest activity against E. faecalis strain, with the diameter of the inhibitory zone reaching 16 ± 0.2 mm at a concentration of 800 μg/mL.These results will be a useful resource for studying the effects of man-made nanoparticles on the environmental safety and represent a promising step toward realizing AgNP-based antimicrobial drug production.

Keywords

• Antibacterial Activity
• AgNPs
• Eco-friendly
• Biosynthesis
• Pathogens
• Blue green algae