Genetic conversion of a split-drive into a full-drive element

Gerard Terradas; Jared B. Bennett; Zhiqian Li; John M. Marshall; Ethan Bier

doi:10.1038/s41467-022-35044-4

Nature Communications (Jan 2023)

Genetic conversion of a split-drive into a full-drive element

Gerard Terradas,
Jared B. Bennett,
Zhiqian Li,
John M. Marshall,
Ethan Bier

Affiliations

Gerard Terradas: Department of Cell and Developmental Biology, University of California, San Diego
Jared B. Bennett: Biophysics Graduate Group, Division of Biological Sciences, College of Letters and Science, University of California
Zhiqian Li: Department of Cell and Developmental Biology, University of California, San Diego
John M. Marshall: Divisions of Epidemiology and Biostatistics, School of Public Health, University of California
Ethan Bier: Department of Cell and Developmental Biology, University of California, San Diego

DOI: https://doi.org/10.1038/s41467-022-35044-4
Journal volume & issue: Vol. 14, no. 1
pp. 1 – 10

Abstract

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CRISPR-based gene-drives can carry the Cas9 and guide RNA (gRNA) components in a single-linked cassette or in separate elements inserted into different genomic loci. Here the authors genetically transform and compare full versus split drives, with the former performing less efficiently than predicted.

Published in Nature Communications

ISSN: 2041-1723 (Online)
Publisher: Nature Portfolio
Country of publisher: United Kingdom
LCC subjects: Science
Website: https://www.nature.com/ncomms/

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