PARP12 is required for mitochondrial function maintenance in thermogenic adipocytes

Fan Hu; Chang Li; Yafen Ye; Xuhong Lu; Miriayi Alimujiang; Ningning Bai; Jingjing Sun; Xiaojing Ma; Xiaohua Li; Ying Yang

doi:10.1080/21623945.2022.2091206

Adipocyte (Dec 2022)

PARP12 is required for mitochondrial function maintenance in thermogenic adipocytes

Fan Hu,
Chang Li,
Yafen Ye,
Xuhong Lu,
Miriayi Alimujiang,
Ningning Bai,
Jingjing Sun,
Xiaojing Ma,
Xiaohua Li,
Ying Yang

Affiliations

Fan Hu: Shanghai Clinical Center for Diabetes, Shanghai Key Clinical Center for Metabolic Disease, Shanghai Diabetes Institute, Shanghai Key Laboratory of Diabetes Mellitus, Shanghai Jiao Tong University Affiliated Sixth People’s Hospital, China
Chang Li: Department of Endocrinology, Seventh People’s Hospital Affiliated to Shanghai University of TCM, Shanghai, China
Yafen Ye: Shanghai Clinical Center for Diabetes, Shanghai Key Clinical Center for Metabolic Disease, Shanghai Diabetes Institute, Shanghai Key Laboratory of Diabetes Mellitus, Shanghai Jiao Tong University Affiliated Sixth People’s Hospital, China
Xuhong Lu: Shanghai Clinical Center for Diabetes, Shanghai Key Clinical Center for Metabolic Disease, Shanghai Diabetes Institute, Shanghai Key Laboratory of Diabetes Mellitus, Shanghai Jiao Tong University Affiliated Sixth People’s Hospital, China
Miriayi Alimujiang: Shanghai Clinical Center for Diabetes, Shanghai Key Clinical Center for Metabolic Disease, Shanghai Diabetes Institute, Shanghai Key Laboratory of Diabetes Mellitus, Shanghai Jiao Tong University Affiliated Sixth People’s Hospital, China
Ningning Bai: Shanghai Clinical Center for Diabetes, Shanghai Key Clinical Center for Metabolic Disease, Shanghai Diabetes Institute, Shanghai Key Laboratory of Diabetes Mellitus, Shanghai Jiao Tong University Affiliated Sixth People’s Hospital, China
Jingjing Sun: Shanghai Clinical Center for Diabetes, Shanghai Key Clinical Center for Metabolic Disease, Shanghai Diabetes Institute, Shanghai Key Laboratory of Diabetes Mellitus, Shanghai Jiao Tong University Affiliated Sixth People’s Hospital, China
Xiaojing Ma: Shanghai Clinical Center for Diabetes, Shanghai Key Clinical Center for Metabolic Disease, Shanghai Diabetes Institute, Shanghai Key Laboratory of Diabetes Mellitus, Shanghai Jiao Tong University Affiliated Sixth People’s Hospital, China
Xiaohua Li: Department of Endocrinology, Seventh People’s Hospital Affiliated to Shanghai University of TCM, Shanghai, China
Ying Yang: Shanghai Clinical Center for Diabetes, Shanghai Key Clinical Center for Metabolic Disease, Shanghai Diabetes Institute, Shanghai Key Laboratory of Diabetes Mellitus, Shanghai Jiao Tong University Affiliated Sixth People’s Hospital, China

DOI: https://doi.org/10.1080/21623945.2022.2091206
Journal volume & issue: Vol. 11, no. 1
pp. 379 – 388

Abstract

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PARP12 is a member of poly-ADP-ribosyl polymerase (PARPs), which has been characterized for its antiviral function. Yet its physiological implication in adipocytes remains unknown. Here, we report a central function of PARP12 in thermogenic adipocytes. We show that PARP12 is highly expressed in brown adipose tissue and is mainly localized to the mitochondria. Knockdown of PARP12 in vitro reduced UCP1 expression. In parallel, the deficiency of PARP12 reduced mitochondrial respiration in adipocytes, while overexpression of PARP12 reversed these effects.

Published in Adipocyte

ISSN: 2162-3945 (Print); 2162-397X (Online)
Publisher: Taylor & Francis Group
Country of publisher: United Kingdom
LCC subjects: Medicine: Internal medicine: Specialties of internal medicine: Diseases of the endocrine glands. Clinical endocrinology; Science: Biology (General): Cytology; Science: Physiology
Website: https://www.tandfonline.com/journals/kadi

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