Química Nova (Jul 2006)

Extração de esterase de fígado suíno (PLE) Pig liver esterase (PLE) extraction

  • Henrique Celso Trevisan,
  • João Batista de Medeiros,
  • Helen Cristina Fávero Lisboa

DOI
https://doi.org/10.1590/S0100-40422006000400040
Journal volume & issue
Vol. 29, no. 4
pp. 865 – 867

Abstract

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A simple, fast and low-cost methodology was optimized, seeking preparation of a crude pig liver esterase (PLE) concentrate. Basically, the method consisted of the following steps: liver homogenization, acetone washing, enzyme extraction and purification/concentration. Starting from 1 kg of fresh liver more than 200 kU of PLE suspension were obtained after 8 hours, at an estimated cost of US$0.21/kU. The PLE concentrate thus obtained was stable, showing 96-100% of the initial activity after 7 months in refrigerator at 4°C.

Keywords