Rapid evaluation of the interaction between bisphenol E and serum albumin by capillary electrophoresis

Abstract

ObjectiveThe interaction between bisphenol E（BPE） and serum albumin was analyzed to provide a useful referrence for the rapid evaluation of the toxicological effect of bisphenols.MethodsElectropherograms of serum albumin with BPE under physiological conditions in vitro were determined using external calibration Hummel-Dreyer （HD） method by capillary electrophoresis. To obtain the interaction parameters， the experimental data were analyzed by three functional forms： Nonlinear equation， Scatchard equation and Klotz equation.ResultsThree form functions were fitted with the interaction parameters. The binding ratio of BPE and serum albumin was about 2. The interaction parameters showed that two kinds of serum albumin had the same number of binding sites， and the binding between different albumin and BPE were both non-covalent interactions.ConclusionAs a simple and effective method， external calibration HD method could be used to analyze the protein pollutant interaction in the food safety field. BPE showed similar moderate interaction with different serum albumin， which suggested that bisphenol compounds had similar pollution distribution regularity in human body and cattle.

Keywords

• capillary electrophoresis
• bisphenol e
• serum albumin
• interaction