A quantitative analysis of various patterns applied in lattice light sheet microscopy

Yu Shi; Timothy A. Daugird; Wesley R. Legant

doi:10.1038/s41467-022-32341-w

Nature Communications (Aug 2022)

A quantitative analysis of various patterns applied in lattice light sheet microscopy

Yu Shi,
Timothy A. Daugird,
Wesley R. Legant

Affiliations

Yu Shi: Joint Department of Biomedical Engineering, University of North Carolina at Chapel Hill, North Carolina State University
Timothy A. Daugird: Department of Pharmacology, University of North Carolina at Chapel Hill
Wesley R. Legant: Joint Department of Biomedical Engineering, University of North Carolina at Chapel Hill, North Carolina State University

DOI: https://doi.org/10.1038/s41467-022-32341-w
Journal volume & issue: Vol. 13, no. 1
pp. 1 – 12

Abstract

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Light sheet microscopes reduce phototoxicity and background while improving imaging speed compared to widefield and confocal microscopes. Here the authors quantify the differences between Gaussian and lattice light sheets using simulations and experimental data in fixed and live cells.

Published in Nature Communications

ISSN: 2041-1723 (Online)
Publisher: Nature Portfolio
Country of publisher: United Kingdom
LCC subjects: Science
Website: https://www.nature.com/ncomms/

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