eLife (May 2016)
Cell type specificity of neurovascular coupling in cerebral cortex
- Hana Uhlirova,
- Kıvılcım Kılıç,
- Peifang Tian,
- Martin Thunemann,
- Michèle Desjardins,
- Payam A Saisan,
- Sava Sakadžić,
- Torbjørn V Ness,
- Celine Mateo,
- Qun Cheng,
- Kimberly L Weldy,
- Florence Razoux,
- Matthieu Vandenberghe,
- Jonathan A Cremonesi,
- Christopher GL Ferri,
- Krystal Nizar,
- Vishnu B Sridhar,
- Tyler C Steed,
- Maxim Abashin,
- Yeshaiahu Fainman,
- Eliezer Masliah,
- Srdjan Djurovic,
- Ole A Andreassen,
- Gabriel A Silva,
- David A Boas,
- David Kleinfeld,
- Richard B Buxton,
- Gaute T Einevoll,
- Anders M Dale,
- Anna Devor
Affiliations
- Hana Uhlirova
- Department of Radiology, University of California, San Diego, La Jolla, United States
- Kıvılcım Kılıç
- Department of Neurosciences, University of California, San Diego, La Jolla, United States
- Peifang Tian
- Department of Neurosciences, University of California, San Diego, La Jolla, United States; Department of Physics, John Carroll University, University Heights, United States
- Martin Thunemann
- Department of Radiology, University of California, San Diego, La Jolla, United States
- Michèle Desjardins
- Department of Radiology, University of California, San Diego, La Jolla, United States
- Payam A Saisan
- Department of Neurosciences, University of California, San Diego, La Jolla, United States
- Sava Sakadžić
- Martinos Center for Biomedical Imaging, Harvard Medical School, Charlestown, United States
- Torbjørn V Ness
- Department of Mathematical Sciences and Technology, Norwegian University of Life Sciences, Ås, Norway
- Celine Mateo
- Department of Physics, University of California, San Diego, La Jolla, United States
- Qun Cheng
- Department of Neurosciences, University of California, San Diego, La Jolla, United States
- Kimberly L Weldy
- Department of Neurosciences, University of California, San Diego, La Jolla, United States
- Florence Razoux
- Department of Neurosciences, University of California, San Diego, La Jolla, United States
- Matthieu Vandenberghe
- Department of Radiology, University of California, San Diego, La Jolla, United States; NORMENT, KG Jebsen Centre for Psychosis Research, Division of Mental Health and Addiction, University of Oslo, Oslo, Norway
- Jonathan A Cremonesi
- Biology Undergraduate Program, University of California, San Diego, La Jolla, United States
- Christopher GL Ferri
- Department of Neurosciences, University of California, San Diego, La Jolla, United States
- Krystal Nizar
- Neurosciences Graduate Program, University of California, San Diego, La Jolla, United States
- Vishnu B Sridhar
- Department of Bioengineering, University of California, San Diego, La Jolla, United States
- Tyler C Steed
- Neurosciences Graduate Program, University of California, San Diego, La Jolla, United States
- Maxim Abashin
- Department of Electrical and Computer Engineering, University of California, San Diego, La Jolla, United States
- Yeshaiahu Fainman
- Department of Electrical and Computer Engineering, University of California, San Diego, La Jolla, United States
- Eliezer Masliah
- Department of Neurosciences, University of California, San Diego, La Jolla, United States
- Srdjan Djurovic
- ORCiD
- Department of Medical Genetics, Oslo University Hospital, Oslo, Norway; NORMENT, KG Jebsen Centre for Psychosis Research, Department of Clinical Science, University of Bergen, Bergen, Norway
- Ole A Andreassen
- NORMENT, KG Jebsen Centre for Psychosis Research, Division of Mental Health and Addiction, University of Oslo, Oslo, Norway
- Gabriel A Silva
- Department of Bioengineering, University of California, San Diego, La Jolla, United States; Department of Ophthalmology, University of California, San Diego, La Jolla, United States
- David A Boas
- Martinos Center for Biomedical Imaging, Harvard Medical School, Charlestown, United States
- David Kleinfeld
- Department of Physics, University of California, San Diego, La Jolla, United States; Department of Electrical and Computer Engineering, University of California, San Diego, La Jolla, United States; Section of Neurobiology, University of California, San Diego, La Jolla, United States
- Richard B Buxton
- Department of Radiology, University of California, San Diego, La Jolla, United States
- Gaute T Einevoll
- ORCiD
- Department of Mathematical Sciences and Technology, Norwegian University of Life Sciences, Ås, Norway; Department of Physics, University of Oslo, Oslo, Norway
- Anders M Dale
- Department of Radiology, University of California, San Diego, La Jolla, United States; Department of Neurosciences, University of California, San Diego, La Jolla, United States
- Anna Devor
- ORCiD
- Department of Radiology, University of California, San Diego, La Jolla, United States; Department of Neurosciences, University of California, San Diego, La Jolla, United States; Martinos Center for Biomedical Imaging, Harvard Medical School, Charlestown, United States
- DOI
- https://doi.org/10.7554/eLife.14315
- Journal volume & issue
-
Vol. 5
Abstract
Identification of the cellular players and molecular messengers that communicate neuronal activity to the vasculature driving cerebral hemodynamics is important for (1) the basic understanding of cerebrovascular regulation and (2) interpretation of functional Magnetic Resonance Imaging (fMRI) signals. Using a combination of optogenetic stimulation and 2-photon imaging in mice, we demonstrate that selective activation of cortical excitation and inhibition elicits distinct vascular responses and identify the vasoconstrictive mechanism as Neuropeptide Y (NPY) acting on Y1 receptors. The latter implies that task-related negative Blood Oxygenation Level Dependent (BOLD) fMRI signals in the cerebral cortex under normal physiological conditions may be mainly driven by the NPY-positive inhibitory neurons. Further, the NPY-Y1 pathway may offer a potential therapeutic target in cerebrovascular disease.
Keywords
