Journal of Pharmacopuncture (Jun 2007)

Expermental Studies of quantitative evaluation using HPLC and safety of Sweet Bee Venom

  • Ki Rok Kwon,
  • Ching Seng Chu,
  • Hee Soo Park,
  • Min Ki Kim,
  • Bae Chun Cha,
  • Eun Lee

DOI
https://doi.org/10.3831/KPI.2007.10.2.081
Journal volume & issue
Vol. 10, no. 2
pp. 81 – 86

Abstract

Read online

Objectives : This study was conducted to carry out quantitative evaluation and safety of Sweet Bee Venom. Methods : Content analysis was done using HPLC, measurement of LD50 was conducted intravenous, subcutaneous, and intra-muscular injection to the ICR mice. Results : 1. According to HPLC analysis, removal of the enzymes containing phospholipase A2 was successfully rendered on Sweet Bee Venom. And analyzing melittin content, Sweet Bee Venom contained 12% more melittin than Bee Venom. 2. LD50 of ICR mice with Sweet Bee Venom was more than 20mg/kg in subcutaneous injection and intravenous injection, between 15mg/kg and 20mg/kg in muscular injection. 3. LD50 of ICR mice with Bee Venom was between 6 and 9mg/kg in subcutaneous injection and intravenous injection, and more than 9mg/kg in muscular injection. Conclusion : Above results indicate that Sweet Bee Venom was more safe than Bee Venom and the process of removing enzymes was well rendered in Sweet Bee Venom.

Keywords