The comparison of the effect of vitamin E on the expression of p53/PTEN of prostate gland of male rats in two groups of intensive continuous and intermittent exercise training

Abstract

Background & Objective: Physical activity and diet are the most important modifiable determinants of cancer risk. The aim of this study was to investigate the comparison of the effect of vitamin E on the expression of p53/PTEN prostate gland of male rats in two groups of intensive continuous and intermittent exercise training. Materials & Methods: For this purpose, 56 male rats were randomly divided into 7 groups: [1] control group, [2] sham group, [3] intensive continuous training, [4] intensive intermittent training, [5] intensive continuous training + vitamin E, [6] intensive intermittent training + vitamin E, [7] vitamin E. The research training protocols were conducted in compliance with the principle of overload on the treadmill for six days a week, lasting 6 weeks. To measure expression changes of p53 and PTEN genes in rats' prostate, real-time PCR method was used and HPLC method was used to measure vitamin E. The One-way analysis of Variance test was used for comparisons among groups. Results: Vitamin E in combination with continuous training induced a significant decrease in the p53 gene expression of IIT + VE group (p > 0/004) compared to the control group. In contrast, vitamin E in combination with intermittent training induced a significant decrease in expression of p53 (p>0/013) and a significant increase in the PTEN gene expression (p>0/035). Conclusion: The results showed that physical exercise training reduced PTEN and p53 tumor suppressing gene expression by reducing oxidative stress, and vitamin E can be a somewhat increased expression of these genes.

Keywords

• vitamin e
• continuous training
• intermittent training
• p53
• pten
• prostate gland