Multimodal multiphoton imaging for label-free monitoring of early gastric cancer
Lianhuang Li,
Deyong Kang,
Zicheng Huang,
Zhenlin Zhan,
Changyin Feng,
Yongjian Zhou,
Haohua Tu,
Shuangmu Zhuo,
Jianxin Chen
Affiliations
Lianhuang Li
Key Laboratory of OptoElectronic Science and Technology for Medicine of Ministry of Education, Fujian Provincial Key Laboratory for Photonics Technology, Fujian Normal University
Deyong Kang
Department of Pathology, Fujian Medical University Union Hospital
Zicheng Huang
Department of Gastroenterology, The First Hospital of Quanzhou Affiliated to Fujian Medical University
Zhenlin Zhan
Key Laboratory of OptoElectronic Science and Technology for Medicine of Ministry of Education, Fujian Provincial Key Laboratory for Photonics Technology, Fujian Normal University
Changyin Feng
Department of Pathology, Fujian Medical University Union Hospital
Yongjian Zhou
Department of Gastric Surgery, Fujian Medical University Union Hospital
Haohua Tu
Beckman Institute for Advanced Science and Technology, University of Illinois at Urbana-Champaign
Shuangmu Zhuo
Key Laboratory of OptoElectronic Science and Technology for Medicine of Ministry of Education, Fujian Provincial Key Laboratory for Photonics Technology, Fujian Normal University
Jianxin Chen
Key Laboratory of OptoElectronic Science and Technology for Medicine of Ministry of Education, Fujian Provincial Key Laboratory for Photonics Technology, Fujian Normal University
Abstract Background Early gastric cancer is associated with a much better prognosis than advanced disease, and strategies to improve prognosis is strictly dependent on earlier detection and accurate diagnosis. Therefore, a label-free, non-invasive imaging technique that allows the precise identification of morphologic changes in early gastric cancer would be of considerable clinical interest. Methods In this study, multiphoton microscopy (MPM) using two-photon excited fluorescence combined with second-harmonic generation was used for the identification of early gastric cancer. Results This microscope was able to directly reveal improved cellular detail and stromal changes during the development of early gastric cancer. Furthermore, two features were quantified from MPM images to assess the cell change in size and stromal collagen change as gastric lesion developed from normal to early cancer. Conclusions These results clearly show that multiphoton microscopy can be used to examine early gastric cancer at the cellular level without the need for exogenous contrast agents. This study would be helpful for early diagnosis and treatment of gastric cancer, and may provide the groundwork for further exploration into the application of multiphoton microscopy in clinical practice.
