Mitochondrial dynamics quantitatively revealed by STED nanoscopy with an enhanced squaraine variant probe

Xusan Yang; Zhigang Yang; Zhaoyang Wu; Ying He; Chunyan Shan; Peiyuan Chai; Chenshuo Ma; Mi Tian; Junlin Teng; Dayong Jin; Wei Yan; Pintu Das; Junle Qu; Peng Xi

doi:10.1038/s41467-020-17546-1

Nature Communications (Jul 2020)

Mitochondrial dynamics quantitatively revealed by STED nanoscopy with an enhanced squaraine variant probe

Xusan Yang,
Zhigang Yang,
Zhaoyang Wu,
Ying He,
Chunyan Shan,
Peiyuan Chai,
Chenshuo Ma,
Mi Tian,
Junlin Teng,
Dayong Jin,
Wei Yan,
Pintu Das,
Junle Qu,
Peng Xi

Affiliations

Xusan Yang: Department of Biomedical Engineering, College of Engineering, Peking University
Zhigang Yang: Key Laboratory of Optoelectronic Devices and Systems of Ministry of Education and Guangdong Province, College of Physics and Optoelectronic Engineering, Shenzhen University
Zhaoyang Wu: Department of Biomedical Engineering, College of Engineering, Peking University
Ying He: Key Laboratory of Optoelectronic Devices and Systems of Ministry of Education and Guangdong Province, College of Physics and Optoelectronic Engineering, Shenzhen University
Chunyan Shan: School of Life Sciences, Peking University
Peiyuan Chai: School of Life Sciences, Peking University
Chenshuo Ma: Material Science and Engineering, Rutgers University
Mi Tian: Key Laboratory of Optoelectronic Devices and Systems of Ministry of Education and Guangdong Province, College of Physics and Optoelectronic Engineering, Shenzhen University
Junlin Teng: School of Life Sciences, Peking University
Dayong Jin: UTS-SUStech Joint Research Centre for Biomedical Materials & Devices, Department of Biomedical Engineering, Southern University of Science and Technology
Wei Yan: Key Laboratory of Optoelectronic Devices and Systems of Ministry of Education and Guangdong Province, College of Physics and Optoelectronic Engineering, Shenzhen University
Pintu Das: Key Laboratory of Optoelectronic Devices and Systems of Ministry of Education and Guangdong Province, College of Physics and Optoelectronic Engineering, Shenzhen University
Junle Qu: Key Laboratory of Optoelectronic Devices and Systems of Ministry of Education and Guangdong Province, College of Physics and Optoelectronic Engineering, Shenzhen University
Peng Xi: Department of Biomedical Engineering, College of Engineering, Peking University

DOI: https://doi.org/10.1038/s41467-020-17546-1
Journal volume & issue: Vol. 11, no. 1
pp. 1 – 9

Abstract

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Live cell imaging of mitochondrial cristae is challenged by the unsuitability of current fluorescent probes and high phototoxicity. Here the authors develop a squarine variant probe (MitoESq-635) that is capable of longitudinal imaging of cristae with STED with minimal phototoxicity.

Published in Nature Communications

ISSN: 2041-1723 (Online)
Publisher: Nature Portfolio
Country of publisher: United Kingdom
LCC subjects: Science
Website: https://www.nature.com/ncomms/

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