Majallah-i dānishgāh-i ̒ulūm-i pizishkī-i Arāk (Jan 2020)
The Effects of Co-Administration of Memantine And Vitamin D on Spatial Learning and Memory Impairment in Adult Male Rats Model of Alzheimer’s Disease
Abstract
Background and Aim: Alzheimer’s disease is the most common causes of dementia among the elderly people. The aim of this study was to evaluate the synergistic effects of memantine and vitamin D on spatial learning and memory impairment in adult male rat model of Alzheimer's disease. Methods & Materials: In this experimental study, male Wistar rats were randomly divided into nine groups (n=7): 1= Control, 2= NBM lesion (received bilateral electric lesion of NBM), 3= Sham (the electrode was entered into the NBM with no electric lesion), 4= NBM lesion+ Vehicle Memantine (received saline), 5= NBM lesion+ Vehicle Vitamin D (received saline), 6= NBM lesion+ Vehicle Memantine+ Vehicle Vitamin D (received saline plus sesame oil), 7= NBM lesion+ Vitamin D; 8= NBM lesion+Memantine, and 9= NBM lesion+Vitamin D+Memantine. After one week, the rats were trained to perform the Y-maze task for five days. Twenty five days after training, a retention test was performed to evaluate their long-term memory. Ethical Considerations: This study with research ethics code of “EE/ 97, 24, 3061243/scu.ac.ir” was approved by the Research Ethics Committee of Shahid Chamran University of Ahvaz In Iran. Results: Bilateral NBM lesion reduced spatial learning in comparison with control and sham groups. No effect on spatial learning was observed in NBM lesion+ Vehicle Memantine and NBM lesion+ Vehicle Vitamin D groups compared to the NBM lesion group. Spatial learning and memory in NBM lesion +Vitamin D+Memantine group (P<0.001) was significantly improved compared to NBM lesion+Vitamin D (P<0.01) and NBM lesion+Memantine (P<0.05) groups. Moreover, no significant difference was observed between the results in the 5th day of training and the memory retention at the 30th day. Conclusion: Co-administration of memantine and vitamin D is more effective than memantine or vitamin D alone in spatial learning and memory improvement in rat model of Alzheimer's disease.
