Allogeneic Adipose-Derived Stem Cells with Low Immunogenicity Constructing Tissue-Engineered Bone for Repairing Bone Defects in Pigs

Ming-Liang Ren; Wei Peng; Zai-Liang Yang; Xin-Jun Sun; Shi-Chang Zhang; Zheng-Guo Wang; Bo Zhang

doi:10.3727/096368912X654966

Cell Transplantation (Dec 2012)

Allogeneic Adipose-Derived Stem Cells with Low Immunogenicity Constructing Tissue-Engineered Bone for Repairing Bone Defects in Pigs

Ming-Liang Ren,
Wei Peng,
Zai-Liang Yang,
Xin-Jun Sun,
Shi-Chang Zhang,
Zheng-Guo Wang,
Bo Zhang

Affiliations

Ming-Liang Ren: Department of Neurosurgery, Research Institute of Surgery and Daping Hospital, Third Military Medical University, Chongqing, China
Wei Peng: Department 4, State Key Laboratory of Trauma, Burn and Combined Injury, Research Institute of Surgery and Daping Hospital, Third Military Medical University, Chongqing, China
Zai-Liang Yang: Department 4, State Key Laboratory of Trauma, Burn and Combined Injury, Research Institute of Surgery and Daping Hospital, Third Military Medical University, Chongqing, China
Xin-Jun Sun: Department 4, State Key Laboratory of Trauma, Burn and Combined Injury, Research Institute of Surgery and Daping Hospital, Third Military Medical University, Chongqing, China
Shi-Chang Zhang: Department 4, State Key Laboratory of Trauma, Burn and Combined Injury, Research Institute of Surgery and Daping Hospital, Third Military Medical University, Chongqing, China
Zheng-Guo Wang: Department 4, State Key Laboratory of Trauma, Burn and Combined Injury, Research Institute of Surgery and Daping Hospital, Third Military Medical University, Chongqing, China
Bo Zhang: Department 4, State Key Laboratory of Trauma, Burn and Combined Injury, Research Institute of Surgery and Daping Hospital, Third Military Medical University, Chongqing, China

DOI: https://doi.org/10.3727/096368912X654966
Journal volume & issue: Vol. 21

Abstract

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The ideal cells for tissue engineering should have the following characteristics: easy obtainment, safety, immune privilege, the capability of self-renewal, and multipotency. Adipose-derived stem cells (ADSCs) are a promising candidate. However, the immunogenicity of allogeneic mesenchymal stem cells limits their long-term benefits. In this study, we introduced human cytomegalovirus US2/US3 gene into the ADSCs to decrease the expression of MHC I protein of ADSCs and reduce the activation of T-cells of the recipient animals. Moreover, the biosafety and biological characteristics of ADSCs transfected with the US2/US3 genes (ADSCs-US2/US3) were similar to normal ADSCs. Then we took ADSCs-US2/US3 to construct a tissue-engineered bone for repairing bone defects in pigs and found that there were no great differences in repair effects or healing time between the allogeneic ADSCs-US2/US3 group and the autologous ADSC group. These results suggest that allogeneic ADSCs-US2/US3 have the advantages of biological safety, low immunogenicity, and effective osteogenesis. Such barely immunogenic ADSCs will be crucial for the success of future tissue-regenerative approaches.

Published in Cell Transplantation

ISSN: 0963-6897 (Print); 1555-3892 (Online)
Publisher: SAGE Publishing
Country of publisher: United States
LCC subjects: Medicine
Website: http://journals.sagepub.com/home/cll

About the journal