Genetic Mapping of a Light-Dependent Lesion Mimic Mutant Reveals the Function of Coproporphyrinogen III Oxidase Homolog in Soybean

Jingjing Ma; Jingjing Ma; Suxin Yang; Dongmei Wang; Dongmei Wang; Kuanqiang Tang; Kuanqiang Tang; Xing Xing Feng; Xing Xing Feng; Xian Zhong Feng

doi:10.3389/fpls.2020.00557

Frontiers in Plant Science (May 2020)

Genetic Mapping of a Light-Dependent Lesion Mimic Mutant Reveals the Function of Coproporphyrinogen III Oxidase Homolog in Soybean

Jingjing Ma,
Jingjing Ma,
Suxin Yang,
Dongmei Wang,
Dongmei Wang,
Kuanqiang Tang,
Kuanqiang Tang,
Xing Xing Feng,
Xing Xing Feng,
Xian Zhong Feng

Affiliations

Jingjing Ma: Key Laboratory of Soybean Molecular Design Breeding, Northeast Institute of Geography and Agroecology, The Innovative Academy of Seed Design, Chinese Academy of Sciences, Changchun, China
Jingjing Ma: University of Chinese Academy of Sciences, Beijing, China
Suxin Yang: Key Laboratory of Soybean Molecular Design Breeding, Northeast Institute of Geography and Agroecology, The Innovative Academy of Seed Design, Chinese Academy of Sciences, Changchun, China
Dongmei Wang: Key Laboratory of Soybean Molecular Design Breeding, Northeast Institute of Geography and Agroecology, The Innovative Academy of Seed Design, Chinese Academy of Sciences, Changchun, China
Dongmei Wang: University of Chinese Academy of Sciences, Beijing, China
Kuanqiang Tang: Key Laboratory of Soybean Molecular Design Breeding, Northeast Institute of Geography and Agroecology, The Innovative Academy of Seed Design, Chinese Academy of Sciences, Changchun, China
Kuanqiang Tang: University of Chinese Academy of Sciences, Beijing, China
Xing Xing Feng: Key Laboratory of Soybean Molecular Design Breeding, Northeast Institute of Geography and Agroecology, The Innovative Academy of Seed Design, Chinese Academy of Sciences, Changchun, China
Xing Xing Feng: University of Chinese Academy of Sciences, Beijing, China
Xian Zhong Feng: Key Laboratory of Soybean Molecular Design Breeding, Northeast Institute of Geography and Agroecology, The Innovative Academy of Seed Design, Chinese Academy of Sciences, Changchun, China

DOI: https://doi.org/10.3389/fpls.2020.00557
Journal volume & issue: Vol. 11

Abstract

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Lesion mimic mutants provide ideal genetic materials for elucidating the molecular mechanism of cell death and disease resistance. Here, we isolated a Glycine max lesion mimic mutant 2-1 (Gmlmm2-1), which displayed a light-dependent cell death phenotype. Map-based cloning revealed that GmLMM2 encods a coproporphyrinogen III oxidase and participates in tetrapyrrole biosynthesis. Knockout of GmLMM2 led to necrotic spots on developing leaves of CRISPR/Cas9 induced mutants. The GmLMM2 defect decreased the chlorophyll content by disrupting tetrapyrrole biosynthesis and enhanced resistance to Phytophthora sojae. These results suggested that GmLMM2 gene played an important role in the biosynthesis of tetrapyrrole and light-dependent defense in soybeans.

Published in Frontiers in Plant Science

ISSN: 1664-462X (Online)
Publisher: Frontiers Media S.A.
Country of publisher: Switzerland
LCC subjects: Agriculture: Plant culture
Website: https://www.frontiersin.org/journals/plant-science

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