Evaluation of Microbial Profile in Patients with Polycystic Ovary Syndrome and Periodontal Disease: A Case-Control Study

Abstract

Background: Polycystic ovary syndrome (PCOS) and oral health are found to share a reciprocal link. Previous substantiatingevidences suggest that PCOS may have a confounding effect on periodontal health and may quantitatively modifythe composition of the oral microbiome. To analyze the role of PCOS as a risk factor in causing periodontal disease, wecompared and evaluated the levels of Porphyromonas gingivalis and Fusobacterium nucleatum in patients with polycysticovary syndrome, polycystic ovary syndrome, chronic periodontitis, polycystic ovary syndrome, and gingivitis, andhealthy controls.Materials and Methods: In this case-control study, 40 female participants are enrolled and grouped into four groupswhich included healthy female individuals, patients with PCOS, patients with PCOS and gingivitis, and patients withPCOS and periodontitis. Periodontal examination is assessed primarily on all the participants using a UNC-15 probe.Dental plaque is then collected using a sterile curette in one stroke and transferred into an Eppendorf tube containingTE Buffer (Tris-EDTA buffer) solution. The level of Porphyromonas gingivalis and Fusobacterium nucleatum wasestimated by real-time polymerase chain reaction (PCR).Results: The levels of Fusobacterium nucleatum were observed to be significantly higher in group with patients withPCOS and periodontitis.Conclusion: PCOS may have an impact on the composition of oral microflora causing repercussions in periodontalhealth.

Keywords

• dental plaque
• periodontitis
• polycystic ovary syndrome