Jichu yixue yu linchuang (May 2024)
BM-MSCs delay the senescence of naive CD8+T cells
Abstract
Objective To verify the effect of bone marrow mesenchymal stem cells (BM-MSCs) in alleviating immune senescence, and to explore the main immune cell population improved by BM-MSCs. Methods Mouse spleen lymphocytes were isolated and stimulated to proliferate for 7 days for constructing a replicative aging model. Flow cytometry was used to detect the p16ink4a(p16) and p21cip1(p21) expression by T cell subpopulation in the young control group, the replicative senescence control group and the BM-MSCs co-cultured group. Results In the replicative senescence model of T lymphocytes, it was observed that CD8+T cells senescent significantly as compared with CD4+T cells after continuous proliferation. Among the naive cells and effector cell subsets of CD8+T cells, effector cell senescence was the most significant. BM-MSCs co-culture had no significant effect on senescent effector cells, and mainly alleviated the senescence of CD8+T cells by delaying the senescence of naive T cells(P<0.01,P<0.001). Conclusions BM-MSCs co-culture can alleviate the replicative senescence phenotype of T cells and has a more significant anti-senescence effect on CD8+T cells by inhibiting the initial senescence of T cells as a major mechcanism.
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