Analysis on Cloning and Expression of Oxidation Resistance Genes in Wax Gourd Under Abiotic Stress

Abstract

【Objective】Identification and cloning of BhiOXR genes from wax gourd and clarification of its expression characteristics under abiotic stresses would lay a foundation for further studying the function of BhiOXR genes in wax gourd.【Method】BhiOXR gene family members from wax gourd reference genome were identified, full-length CDS primers were designed to clone the BhiOXR genes of the wax gourd by RT-PCR. Seedlings of wax gourd inbred line B227 were treated with low temperature(4℃), high temperature(40℃), salt stress(150 mmol/L NaCl solution) and drought(10% PEG6000 solution).The expression characteristics of BhiOXR genes under the above mentioned abiotic stresses treatment time: 0, 4, 8, 12, 24 h were analyzed by using qRT-PCR.【Result】Six BhiOXR members were identified from wax gourd reference genome, and the fulllength genes were further cloned and bioinformatically analyzed. qRT-PCR analysis results showed that BhiOXR1 responded to low temperature, high temperature and drought stress, but did not respond to salt stress. BhiOXR2a, BhiOXR2b and BhiOXR4 responded to low temperature, salt and drought stresses but not to high temperature stress; BhiOXR3 and BhiOXR5 responded to low temperature, high temperature and salt stresses but not to drought stress.【Conclusion】It is clarified that BhiOXR genes are induced by abiotic stresses as low temperature, high temperature, salt and drought, and different BhiOXR genes have differences in response to abiotic stresses. Therefore, it is speculated that the six BhiOXR members may have different antioxidant effects in the process of resisting different abiotic stresses, which lays a foundation for further exploring the biological functions of BhiOXR genes in wax gourd.

Keywords

• wax gourd
• oxidation resistance genes(oxr)
• abiotic stress
• expression characteristic