Transcriptome Analysis of Immune Responses and Metabolic Regulations of Chinese Soft-Shelled Turtle (<i>Pelodiscus sinensis</i>) against <i>Edwardsiella tarda</i> Infection
Qianrong Liang,
Ningyu Zhu,
Xiaoye Zheng,
Xueyan Ding,
Runzhen He,
Hongsen Xu,
Feifei Cao,
Huili Xue,
Fan Zhou,
Tianlun Zheng
Affiliations
Qianrong Liang
Zhejiang Fisheries Technical Extension Center, Zhejiang Fisheries Test and Aquatic Disease Prevention Center, No.181 Jingchang Road, Hangzhou 310023, China
Ningyu Zhu
Zhejiang Fisheries Technical Extension Center, Zhejiang Fisheries Test and Aquatic Disease Prevention Center, No.181 Jingchang Road, Hangzhou 310023, China
Xiaoye Zheng
Zhejiang Fisheries Technical Extension Center, Zhejiang Fisheries Test and Aquatic Disease Prevention Center, No.181 Jingchang Road, Hangzhou 310023, China
Xueyan Ding
Zhejiang Fisheries Technical Extension Center, Zhejiang Fisheries Test and Aquatic Disease Prevention Center, No.181 Jingchang Road, Hangzhou 310023, China
Runzhen He
Zhejiang Fisheries Technical Extension Center, Zhejiang Fisheries Test and Aquatic Disease Prevention Center, No.181 Jingchang Road, Hangzhou 310023, China
Hongsen Xu
Hubei Key Laboratory of Animal Nutrition and Feed Science, School of Animal Science and Nutritional Engineering, Wuhan Polytechnic University, Wuhan 430023, China
Feifei Cao
Zhejiang Fisheries Technical Extension Center, Zhejiang Fisheries Test and Aquatic Disease Prevention Center, No.181 Jingchang Road, Hangzhou 310023, China
Huili Xue
Zhejiang Fisheries Technical Extension Center, Zhejiang Fisheries Test and Aquatic Disease Prevention Center, No.181 Jingchang Road, Hangzhou 310023, China
Fan Zhou
Zhejiang Fisheries Technical Extension Center, Zhejiang Fisheries Test and Aquatic Disease Prevention Center, No.181 Jingchang Road, Hangzhou 310023, China
Tianlun Zheng
Zhejiang Fisheries Technical Extension Center, Zhejiang Fisheries Test and Aquatic Disease Prevention Center, No.181 Jingchang Road, Hangzhou 310023, China
The Chinese soft-shelled turtle (Pelodiscus sinensis) is an important aquatic species in southern China that is threatened by many serious diseases. Edwardsiella tarda is one of the highly pathogenic bacteria that cause the white abdominal shell disease. Yet, little is known about the immune and metabolic responses of the Chinese soft-shelled turtle against E. tarda infection. In the paper, gene expression profiles in the turtle liver were obtained to study the immune responses and metabolic regulations induced by E. tarda infection using RNA sequencing. A total of 3908 differentially expressed unigenes between the experimental group and the control group were obtained by transcriptome analysis, among them, were the significantly upregulated unigenes and downregulated unigenes 2065 and 1922, respectively. Further annotation and analysis revealed that the DEGs were mainly enriched in complement and coagulation cascades, phagosome, and steroid hormone biosynthesis pathways, indicating that they were mainly associated with defense mechanisms in the turtle liver against E. tarda four days post infection. For the first time, we reported on the gene profile of anti-E. tarda response in the soft-shelled turtle, and our research might provide valuable data to support further study on anti-E. tarda defense mechanisms in turtles.
