New Prospects in Neutering Male Animals Using Magnetic Nanoparticle Hyperthermia
José Luiz P. R. Jivago,
Juliana Lis Mendes Brito,
Gustavo Capistrano,
Marcus Vinícius-Araújo,
Ediron Lima Verde,
Andris Figueiroa Bakuzis,
Paulo E. N. Souza,
Ricardo Bentes Azevedo,
Carolina Madeira Lucci
Affiliations
José Luiz P. R. Jivago
Laboratory of Animal Reproduction, Department of Physiological Sciences, Institute of Biological Sciences, Campus Universitário Darcy Ribeiro, Brasilia 70910-900, DF, Brazil
Juliana Lis Mendes Brito
Laboratory of Animal Reproduction, Department of Physiological Sciences, Institute of Biological Sciences, Campus Universitário Darcy Ribeiro, Brasilia 70910-900, DF, Brazil
Gustavo Capistrano
Institute of Physics and CNanoMed, Federal University of Goiás, Goiania 74884-092, GO, Brazil
Marcus Vinícius-Araújo
Institute of Physics and CNanoMed, Federal University of Goiás, Goiania 74884-092, GO, Brazil
Ediron Lima Verde
Instituto de Ciências Exatas e da Terra, Universidade Federal de Mato Grosso, Pontal do Araguaia 78060-900, MT, Brazil
Andris Figueiroa Bakuzis
Institute of Physics and CNanoMed, Federal University of Goiás, Goiania 74884-092, GO, Brazil
Paulo E. N. Souza
Laboratory of Electron Paramagnetic Resonance, Institute of Physics, University of Brasilia, Brasilia 70910-900, DF, Brazil
Ricardo Bentes Azevedo
Department of Genetics and Morphology, Institute of Biological Sciences, Campus Universitário Darcy Ribeiro, Brasilia 70910-900, DF, Brazil
Carolina Madeira Lucci
Laboratory of Animal Reproduction, Department of Physiological Sciences, Institute of Biological Sciences, Campus Universitário Darcy Ribeiro, Brasilia 70910-900, DF, Brazil
Controlling populations of free-roaming dogs and cats poses a huge challenge worldwide. Non-surgical neutering strategies for male animals have been long pursued, but the implementation of the procedures developed has remained limited to date. As submitting the testes to high temperatures impairs spermatogenesis, the present study investigated localized application of magnetic nanoparticle hyperthermia (MNH) to the testicles as a potential non-surgical sterilization method for animals. An intratesticular injection of a magnetic fluid composed of manganese-ferrite nanoparticles functionalized with citrate was administered followed by testicle exposure to an alternate magnetic field to generate localized heat. Testicular MNH was highly effective, causing progressive seminiferous tubule degeneration followed by substitution of the parenchyma with stromal tissue and gonadal atrophy, suggesting an irreversible process with few side effects to general animal health.
