Stem Cell Research (Mar 2016)

Derivation of DM1 affected human embryonic stem cell line Genea067

  • Biljana Dumevska,
  • Heather Main,
  • Robert McKernan,
  • Divya Goel,
  • Uli Schmidt

DOI
https://doi.org/10.1016/j.scr.2016.02.014
Journal volume & issue
Vol. 16, no. 2
pp. 437 – 439

Abstract

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The Genea067 human embryonic stem cell line was derived from a donated, fully commercially consented ART blastocyst, carrying expansion of CTG repeats in the DMPK gene, indicative of Myotonic Dystrophy Type 1 (DM1). Following ICM outgrowth on inactivated human feeders, karyotype was confirmed as 46, XY and STR analysis demonstrated a male Allele pattern. The hESC line had pluripotent cell morphology, 85% of cells expressed Nanog, 97% Oct4, 73% Tra1-60 and 98% SSEA4 and gave a Pluritest Pluripotency score of 25.75, Novelty of 1.46. The cell line was negative for Mycoplasma and visible contamination.