DEVELOPMENT AND IMPROVEMENT OF ASF SEROLOGICAL DIAGNOSTIC TECHNIQUES

Abstract

Due to the lack of effective tools of ASF specific prevention it is evident that early diagnosis is one of the most important and resultative ways of the disease control. However, contemporary diagnosis is a complex component of any effective surveillance system. Latest scientific achievements facilitated not only highly specific and sensitive but also rapid methods of laboratory diagnosis. Nevertheless, further development, improvement and expansion of ASF diagnosis techniques including rapid tests is a topical task of a great concern. The research is devoted to development of rapid test methods for rapid detection of antibodies to ASFV in blood sera of infected animals as well as to analysis of their use effectiveness. The following methods were suggested: immunoperoxidase monolayer assay using fixed cell line (ASFV permissive CV-1 cell-line infected with the virus strain ASF/ARRIAH/CV-1) and latex agglutination test using ASFV p30 recombinant protein. The performed research demonstrated the effectiveness of the applied techniques for ASF serological diagnosis. Latex agglutination test and immunoperoxidase monolayer assay give rapid and high quality test results (within 1–2 hours). The advantage of the specified methods as compared to ELISA is their simplicity and the possibility of use in conditions of limited technical support.

Keywords

• african swine fever
• latex agglutination test
• immunoperoxidase monolayer assay
• cv-1 continuous cell line
• p30 recombinant protein
• virus antigen