Different Oxidative Stress and Inflammation Patterns of Diseased Left Anterior Descending Coronary Artery versus Internal Thoracic Artery
Andrea Salica,
Vittoria Cammisotto,
Raffaele Scaffa,
Giulio Folino,
Ruggero De Paulis,
Roberto Carnevale,
Umberto Benedetto,
Wael Saade,
Antonino Marullo,
Sebastiano Sciarretta,
Gianmarco Sarto,
Silvia Palmerio,
Valentina Valenti,
Mariangela Peruzzi,
Fabio Miraldi,
Francesco Giosuè Irace,
Giacomo Frati
Affiliations
Andrea Salica
Department of Cardiac Surgery, European Hospital, 00149 Rome, Italy
Vittoria Cammisotto
Department of Clinical, Internal Anesthesiological and Cardiovascular Sciences, Sapienza University of Rome, Viale del Policlinico, 155, 00161 Rome, Italy
Raffaele Scaffa
Department of Cardiac Surgery, European Hospital, 00149 Rome, Italy
Giulio Folino
Department of Cardiac Surgery, European Hospital, 00149 Rome, Italy
Ruggero De Paulis
Department of Cardiac Surgery, European Hospital, 00149 Rome, Italy
Roberto Carnevale
Department of Medico-Surgical Sciences and Biotechnologies, Sapienza University of Rome, Corso della Repubblica 79, 04100 Latina, Italy
Umberto Benedetto
Department of Cardiac Surgery, University “G. d’Annunzio”, 66013 Pescara, Italy
Wael Saade
Department of Clinical, Internal Anesthesiological and Cardiovascular Sciences, Sapienza University of Rome, Viale del Policlinico, 155, 00161 Rome, Italy
Antonino Marullo
Department of Medico-Surgical Sciences and Biotechnologies, Sapienza University of Rome, Corso della Repubblica 79, 04100 Latina, Italy
Sebastiano Sciarretta
Department of Medico-Surgical Sciences and Biotechnologies, Sapienza University of Rome, Corso della Repubblica 79, 04100 Latina, Italy
Gianmarco Sarto
Department of Medico-Surgical Sciences and Biotechnologies, Sapienza University of Rome, Corso della Repubblica 79, 04100 Latina, Italy
Silvia Palmerio
IRCCS NeuroMed, 86077 Pozzilli, Italy
Valentina Valenti
Department of Medico-Surgical Sciences and Biotechnologies, Sapienza University of Rome, Corso della Repubblica 79, 04100 Latina, Italy
Mariangela Peruzzi
Department of Clinical, Internal Anesthesiological and Cardiovascular Sciences, Sapienza University of Rome, Viale del Policlinico, 155, 00161 Rome, Italy
Fabio Miraldi
Department of Clinical, Internal Anesthesiological and Cardiovascular Sciences, Sapienza University of Rome, Viale del Policlinico, 155, 00161 Rome, Italy
Francesco Giosuè Irace
Department of Cardiac Surgery and Heart Transplantation, San Camillo Forlanini Hospital, Circ.ne Gianicolense 87, 00152 Rome, Italy
Giacomo Frati
Department of Medico-Surgical Sciences and Biotechnologies, Sapienza University of Rome, Corso della Repubblica 79, 04100 Latina, Italy
Background. Oxidative stress and inflammation are typically implied in atherosclerosis pathogenesis and progression, especially in coronary artery disease (CAD). Our objective was to investigate the oxidative stress and inflammation burden directly associated with atherosclerotic plaque in patients with stable coronary disease undergoing coronary artery bypass graft (CABG) surgery. Specifically, markers of oxidative stress and inflammation were compared in blood samples obtained from the atherosclerotic left anterior descending artery (LAD) and blood samples obtained from the healthy left internal thoracic artery (LITA), used as a bypass graft, within the same patient. Methods. Twenty patients scheduled for off-pump CABG were enrolled. Blood samples were collected from the LITA below anastomosis and the LAD below the stenosis. Samples were analysed for oxidative stress (sNOXdp, H2O2, NO) and inflammation markers (TNFα, IL-6, IL-1β, IL-10). Results. The analysis showed a significant increase in oxidative stress burden in the LAD as compared to LITA, as indicated by higher sNOX2-dp and H2O2 levels and lower NO levels (p p < 0.01). On the other hand, no significant differences could be seen regarding IL-1β and IL-10 levels between the two groups. Conclusions. The oxidative stress and inflammatory burden are specifically enhanced in the LAD artery of stable coronary patients compared to systemic blood from the LITA of stable coronary patients.
