Infection and Drug Resistance (Mar 2022)
Association of Mannose-Binding Lectin 2 Gene Polymorphism with Tuberculosis Based on Mycobacterium tuberculosis Lineages
Abstract
Mengwen Liu,1 Quan Wang,2 Haican Liu,3 Chunjie Yin,1 Xiaokaiti Mijiti,2 Aiketaguli Anwaierjiang,1 Kanglin Wan,3 Miao Xu,2 Machao Li,3 Siqin Nong,4 Guilian Li,3 Hui Xiao1 1School of Public Health, Xinjiang Medical University, Urumqi, 830011, People’s Republic of China; 2The Eighth Affiliated Hospital of Xinjiang Medical University, Urumqi, 830001, People’s Republic of China; 3State Key Laboratory for Infectious Disease Prevention and Control, Collaborative Innovation Center for Diagnosis and Treatment of Infectious Diseases, National Institute for Communicable Disease Control and Prevention, Chinese Center for Disease Control and Prevention, Beijing, 102206, People’s Republic of China; 4College of Life Science and Technology, Beijing University of Chemical Technology, Beijing, 100029, People’s Republic of ChinaCorrespondence: Guilian Li; Hui Xiao, Email [email protected]; [email protected]: Polymorphisms in MBL2 may contribute to the susceptibility to tuberculosis. The aim of the present study was to determine the associations of the polymorphisms of five loci (rs1800450, rs1800451, rs7096206, rs7095891, and rs11003125) in the MBL2 gene with susceptibility to tuberculosis and specific lineages of Mycobacterium tuberculosis causing tuberculosis in the Uyghur population of Xinjiang, China.Methods: From January 2019 to January 2020, we enrolled 170 Uyghur tuberculosis patients as the case group and 147 Uyghur staff with no clinical symptoms as the control group from four designated tuberculosis hospitals in southern Xinjiang, China. The polymorphisms of five loci in MBL2 of human were detected by sequencing. Whole-genome sequencing was applied in 68 M. tuberculosis isolates from the case group and the data were used to perform genealogy analysis.Results: The distributions of allele and genotype frequencies of five loci in MBL2 varied little between the case and control groups and varied little among the groups, including those infected with different lineages of M. tuberculosis and the control (except those of rs11003125), the P values were all > 0.05. The distribution of alleles of rs11003125 was statistically different between patients infected with lineages 3 and 4 M. tuberculosis (χ2=7.037, P=0.008). The C allele and CC genotype of rs11003125 were found to be protective factors against lineage 4 infection when compared to lineage 3 (ORs were 0.190 and 0.158, respectively; 95% confidence intervals were 0.053∼ 0.690 and 0.025∼ 0.999, respectively).Conclusion: Our results suggested that human’s susceptibility to tuberculosis is affected both by the host genetic polymorphisms and the lineage of the M. tuberculosis that people were exposed to. However, due to the limitation of the sample size in the present study, larger sample size and more rigorous design should be guaranteed in future studies.Keywords: mannose-binding lectin 2 gene, polymorphism, tuberculosis, Mycobacterium tuberculosis, lineage
