Studies on developmental variation of isoperoxidase and protein profile of Zea mays L.

Abstract

The present study was aimed to produce the isoperoxidase and protein marker for the maize cultivars from India and to find the isoperoxidase and protein expression on the various developmental stages. The leaf samples of Zea mays L. were harvested on 3rd, 7th, 11th, 15th, 19th, 23rd and 27th d for electrophoretic analysis of isoperoxidase and protein. The total protein and isoperoxidase were isolated using the standard procedure described by Sadasivam and Manickam. Isozyme and protein separation was carried out using 10% non-denaturing polyacrylamide gel electrophoresis. The banding pattern suggested several similarities as well as presence of unique isoperoxidase and protein in each stage. These studies will be useful for the identification of the major proteins present at different developmental stages of Z. mays.

Keywords

• Electrophoresis
• isoperoxidase
• protein
• Zea mays