Rapid Detection of Carbapenemase-producing Enterobacteriaceae

Abstract

To rapidly identify carbapenemase producers in Enterobacteriaceae, we developed the Carba NP test. The test uses isolated bacterial colonies and is based on in vitro hydrolysis of a carbapenem, imipenem. It was 100% sensitive and specific compared with molecular-based techniques. This rapid (<2 hours), inexpensive technique may be implemented in any laboratory.

Keywords

• antimicrobial drug resistance
• antibiotic
• gram-negatives bacteria
• ß-lactamase
• carbapenemase
• β-lactamase