Where and When Bacterial Chromosome Replication Starts: A Single Cell Perspective

Abstract

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Bacterial chromosomes have a single, unique replication origin (named oriC), from which DNA synthesis starts. This study describes methods of visualizing oriC regions and the chromosome replication in single living bacterial cells in real-time. This review also discusses the impact of live cell imaging techniques on understanding of chromosome replication dynamics, particularly at the initiation step, in different species of bacteria.

Keywords

• replication initiation
• oriC
• replisome
• single-cell
• bacterial chromosome