Nature Communications (Mar 2023)
Reference compounds for characterizing cellular injury in high-content cellular morphology assays
- Jayme L. Dahlin,
- Bruce K. Hua,
- Beth E. Zucconi,
- Shawn D. Nelson,
- Shantanu Singh,
- Anne E. Carpenter,
- Jonathan H. Shrimp,
- Evelyne Lima-Fernandes,
- Mathias J. Wawer,
- Lawrence P. W. Chung,
- Ayushi Agrawal,
- Mary O’Reilly,
- Dalia Barsyte-Lovejoy,
- Magdalena Szewczyk,
- Fengling Li,
- Parnian Lak,
- Matthew Cuellar,
- Philip A. Cole,
- Jordan L. Meier,
- Tim Thomas,
- Jonathan B. Baell,
- Peter J. Brown,
- Michael A. Walters,
- Paul A. Clemons,
- Stuart L. Schreiber,
- Bridget K. Wagner
Affiliations
- Jayme L. Dahlin
- National Center for Advancing Translational Sciences, National Institutes of Health
- Bruce K. Hua
- Chemical Biology and Therapeutics Science Program, Broad Institute
- Beth E. Zucconi
- Division of Genetics, Departments of Medicine and Biological Chemistry and Molecular Pharmacology, Harvard Medical School and Brigham and Women’s Hospital
- Shawn D. Nelson
- Stanley Center, Broad Institute
- Shantanu Singh
- Imaging Platform, Broad Institute
- Anne E. Carpenter
- Imaging Platform, Broad Institute
- Jonathan H. Shrimp
- Chemical Biology Laboratory, Center for Cancer Research, National Cancer Institute, National Institutes of Health
- Evelyne Lima-Fernandes
- Structural Genomics Consortium, University of Toronto
- Mathias J. Wawer
- Chemical Biology and Therapeutics Science Program, Broad Institute
- Lawrence P. W. Chung
- Chemical Biology and Therapeutics Science Program, Broad Institute
- Ayushi Agrawal
- Chemical Biology and Therapeutics Science Program, Broad Institute
- Mary O’Reilly
- Pattern, Broad Institute
- Dalia Barsyte-Lovejoy
- Structural Genomics Consortium, University of Toronto
- Magdalena Szewczyk
- Structural Genomics Consortium, University of Toronto
- Fengling Li
- Structural Genomics Consortium, University of Toronto
- Parnian Lak
- Department of Pharmaceutical Chemistry and Quantitative Biology Institute, University of California San Francisco
- Matthew Cuellar
- Institute for Therapeutics Discovery and Development, University of Minnesota
- Philip A. Cole
- Division of Genetics, Departments of Medicine and Biological Chemistry and Molecular Pharmacology, Harvard Medical School and Brigham and Women’s Hospital
- Jordan L. Meier
- Chemical Biology Laboratory, Center for Cancer Research, National Cancer Institute, National Institutes of Health
- Tim Thomas
- Department of Medical Biology, University of Melbourne
- Jonathan B. Baell
- Medicinal Chemistry Theme, Monash Institute of Pharmaceutical Sciences, Monash University
- Peter J. Brown
- Structural Genomics Consortium, University of Toronto
- Michael A. Walters
- Institute for Therapeutics Discovery and Development, University of Minnesota
- Paul A. Clemons
- Chemical Biology and Therapeutics Science Program, Broad Institute
- Stuart L. Schreiber
- Chemical Biology and Therapeutics Science Program, Broad Institute
- Bridget K. Wagner
- Chemical Biology and Therapeutics Science Program, Broad Institute
- DOI
- https://doi.org/10.1038/s41467-023-36829-x
- Journal volume & issue
-
Vol. 14,
no. 1
pp. 1 – 16
Abstract
Cellular nuisance compounds are a burden in chemical biology and drug screening. Here the authors profile prototypical cytotoxic and nuisance compounds using the cell painting assay to systematically characterise cellular morphologies associated with compound-dependent cellular injury and nuisance activity.
