Research Progress on Alternative Methods of Skin Sensitization Test

Abstract

Allergic contact dermatitis is a type Ⅳ hypersensitivity reaction caused by repeated skin exposure to a substance and is a common public health problem. Traditional skin sensitization tests are based on animal experiments such as guinea pig maximum test and closed patch. In recent years, with the increasing attention of animal ethics and the development of science and technology, alternative methods of skin sensitization test have emerged. According to different principles, these alternative methods are divided into in vivo alternative methods, several in vitro alternative methods based on harmful outcome pathways, and genomic allergen rapid test, etc. In this paper, we reviewed the progress of these alternative methods of skin sensitization test, and several integrated testing and evaluation methods based on adverse outcome pathways.

Keywords

• skin sensitization experiment
• allergic contact dermatitis
• alternative method