Emerging Infectious Diseases (Jan 2019)

Dengue Virus IgM Serotyping by ELISA with Recombinant Mutant Envelope Proteins

  • Alexandra Rockstroh,
  • Luisa Barzon,
  • Widuranga Kumbukgolla,
  • Hoang Xuan Su,
  • Erley Lizarazo,
  • Maria Fernanda Vincenti-Gonzalez,
  • Adriana Tami,
  • Alice M.M. Ornelas,
  • Renato Santana Aguiar,
  • Daniel Cadar,
  • Jonas Schmidt-Chanasit,
  • Sebastian Ulbert

DOI
https://doi.org/10.3201/eid2501.180605
Journal volume & issue
Vol. 25, no. 1
pp. 1111 – 1115

Abstract

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We developed an IgM-based ELISA that identifies the dengue virus serotype of recent infections. Dominant serotypes were detectable in 91.1% of samples from travelers and 86.5% of samples from residents of endemic regions; 97.1% corresponded to the serotype identified by PCR. This ELISA enables more accurate reporting of epidemiologic findings.

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