Discovery of bioactive compounds in the Chinese herbal formula NaoShuanTong Capsule (NSTC) against hemorheological disorders

Abstract

NaoShuanTong capsule (NSTC) has been commonly used to treat blood circulation disorders and neurological dysfunction against cerebral ischemia in China. In this study, using an acute blood stasis rat model, canonical correlation analysis between chemical high performance liquid chromatography (HPLC) fingerprint and in vivo biotests was conducted to discover and identify the bioactive compounds responsible for NSTC’s protective effects on hemorheological disorders. Four independent effect components were extracted from the 12 originally detected parameters. Three bioactive compounds were found to have significant contribution in two or more effect components against hemorheological disorders. Typhaneoside could influence the erythrocyte aggregation and coagulation function. Isorhamnetin-3-O-neohesperidoside had impacts on the erythrocyte aggregation, erythrocyte deformability and platelet aggregation. Benzoyl-oxypaeoniflorin was associated with erythrocyte aggregation, coagulation function and platelet aggregation. The findings could offer useful references for NSTC’s quality control and clinical practice. Abbreviations Adradrenaline ASPaspirin APTTactivated partial thromboplastin time CCAcanonical correlation analysis EAIerythrocyte aggregation index EDIerythrocyte deformability index EEIerythrocyte electrophoresis index HCThaematokrit HPLChigh performance liquid chromatography MPARmaximum platelet aggregation rate NSTCNaoShuanTong Capsule PCAprincipal component analysis PTPollen Typhae PTprothrombin time PVplasma viscosity RCRadix Curcumae RGRhizome Gastrodiae RPRRadix Paeoniae Rubra RRRadix Rhapontici TCMtraditional Chinese medicine WBVwhole blood viscosit

Keywords

• NaoShuanTong capsule (NSTC)
• bioactive compounds
• hemorheological disorders
• canonical correlation analysis
• in vivo biotests