Frontiers in Immunology (Jun 2019)

Single-Cell High-Throughput Technologies in Cerebrospinal Fluid Research and Diagnostics

  • Tobias V. Lanz,
  • Tobias V. Lanz,
  • Anne-Katrin Pröbstel,
  • Anne-Katrin Pröbstel,
  • Iris Mildenberger,
  • Michael Platten,
  • Michael Platten,
  • Lucas Schirmer

DOI
https://doi.org/10.3389/fimmu.2019.01302
Journal volume & issue
Vol. 10

Abstract

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High-throughput single-cell technologies have recently emerged as essential tools in biomedical research with great potential for clinical pathology when studying liquid and solid biopsies. We provide an update on current single-cell methods in cerebrospinal fluid research and diagnostics, focusing on high-throughput cell-type specific proteomic and genomic technologies. Proteomic methods comprising flow cytometry and mass cytometry as well as genomic approaches including immune cell repertoire and single-cell transcriptomic studies are critically reviewed and future directions discussed.

Keywords