Establishment of IGF-1 and IGFBP-3 continuous reference percentiles from data of healthy children using three kinds of immunoassay systems
Yongseok Jo,
Kyungchul Song,
Seok-Jae Heo,
Junghwan Suh,
Hyun Wook Chae,
John Hoon Rim,
Yongjung Park,
Jong Baek Lim,
Ho-Seong Kim,
Jeong-Ho Kim
Affiliations
Yongseok Jo
Department of Laboratory Medicine, Yongin Severance Hospital, Yonsei University College of Medicine, Yongin, South Korea; Department of Laboratory Medicine, Severance Hospital, Yonsei University College of Medicine, Seoul, South Korea
Kyungchul Song
Department of Pediatrics, Yonsei University College of Medicine, Seoul, South Korea
Seok-Jae Heo
Department of Biomedical Systems Informatics, Yonsei University College of Medicine, Seoul, South Korea
Junghwan Suh
Department of Pediatrics, Yonsei University College of Medicine, Seoul, South Korea
Hyun Wook Chae
Department of Pediatrics, Yonsei University College of Medicine, Seoul, South Korea
John Hoon Rim
Department of Laboratory Medicine, Severance Hospital, Yonsei University College of Medicine, Seoul, South Korea
Yongjung Park
Department of Laboratory Medicine, Yongin Severance Hospital, Yonsei University College of Medicine, Yongin, South Korea
Jong Baek Lim
Department of Laboratory Medicine, Severance Hospital, Yonsei University College of Medicine, Seoul, South Korea
Ho-Seong Kim
Department of Pediatrics, Yonsei University College of Medicine, Seoul, South Korea; Corresponding author. Department of Pediatrics, Yonsei University College of Medicine, 50-1 Yonsei-ro, Seodaemun-gu, Seoul, 03722, South Korea.
Jeong-Ho Kim
Department of Laboratory Medicine, Yongin Severance Hospital, Yonsei University College of Medicine, Yongin, South Korea; Corresponding author. Department of Laboratory Medicine, Yonsei University College of Medicine, South Korea.
Background and aims: Appropriate continuous reference intervals (RIs) for serum insulin-like growth factor 1 (IGF-1) and insulin-like growth factor-binding protein 3 (IGFBP-3) are important for diagnosing growth hormone deficiency or excess. Material and methods: We retrospectively reviewed serum IGF-1 and IGFBP-3 levels in Korean children aged 0–17 years who were diagnosed as healthy during a short stature workup in the outpatient clinics of three hospitals. IGF-1 and IGFBP-3 levels were measured using various immunoassays, including Liaison XL for IGF-1, an immunoradiometric assay (IRMA) for IGFBP-3 (n = 5522), and Immulite 2000 (n = 3036) and cobas e801 (n = 314). We established RIs from the 2.5th to 97.5th percentile RI curves using the lambda–mu–sigma (LMS) method for each sex group. Results: Pediatric serum continuous IGF-1 and IGFBP-3 reference percentiles by LMS method were found to be immunoassay method-dependent, but aligned relatively well with the manufacturers’ RIs. IGFBP-3 levels displayed notable discrepancies among the different immunoassay methods. Conclusion: Age- and sex-specific pediatric LMS based continuous reference intervals are method dependent and they should be calculated for dynamic parameters that show variations throughout childhood.
