Identification of DNA Methylation Differences in Pituitary Tissues of Sichuan White Geese Using Whole-Genome Bisulfite Sequencing (WGBS)
Lin Ma,
Xianzhi Zhao,
Guoda A,
Tongtong Song,
Meng Wu,
Zhihao Yan,
Min Xiao,
Wenbo Jiang,
Yixiao Gao,
Haiwei Wang,
Zhuping Chen,
Keshan Zhang,
Jiajia Xue,
Yi Luo,
Chao Wang,
Youhui Xie,
Ying Chen,
Guangliang Gao,
Qigui Wang
Affiliations
Lin Ma
Chongqing Engineering Research Center of Goose Genetic Improvement, Institute of Poultry Science, Chongqing Academy of Animal Science, Chongqing 402460, China
Xianzhi Zhao
Chongqing Engineering Research Center of Goose Genetic Improvement, Institute of Poultry Science, Chongqing Academy of Animal Science, Chongqing 402460, China
Guoda A
College of Animal Sciences and Technology, Northeast Agricultural University, Harbin 150030, China
Tongtong Song
College of Animal Sciences and Technology, Northeast Agricultural University, Harbin 150030, China
Meng Wu
College of Animal Science and Technology, Southwest University, Chongqing 402460, China
Zhihao Yan
College of Animal Science and Technology, Southwest University, Chongqing 402460, China
Min Xiao
College of Animal Science and Technology, Southwest University, Chongqing 402460, China
Wenbo Jiang
College of Animal Science and Technology, Southwest University, Chongqing 402460, China
Yixiao Gao
College of Animal Science and Technology, Southwest University, Chongqing 402460, China
Haiwei Wang
Chongqing Engineering Research Center of Goose Genetic Improvement, Institute of Poultry Science, Chongqing Academy of Animal Science, Chongqing 402460, China
Zhuping Chen
Chongqing Engineering Research Center of Goose Genetic Improvement, Institute of Poultry Science, Chongqing Academy of Animal Science, Chongqing 402460, China
Keshan Zhang
Chongqing Engineering Research Center of Goose Genetic Improvement, Institute of Poultry Science, Chongqing Academy of Animal Science, Chongqing 402460, China
Jiajia Xue
Chongqing Engineering Research Center of Goose Genetic Improvement, Institute of Poultry Science, Chongqing Academy of Animal Science, Chongqing 402460, China
Yi Luo
Chongqing Engineering Research Center of Goose Genetic Improvement, Institute of Poultry Science, Chongqing Academy of Animal Science, Chongqing 402460, China
Chao Wang
Chongqing Engineering Research Center of Goose Genetic Improvement, Institute of Poultry Science, Chongqing Academy of Animal Science, Chongqing 402460, China
Youhui Xie
Chongqing Engineering Research Center of Goose Genetic Improvement, Institute of Poultry Science, Chongqing Academy of Animal Science, Chongqing 402460, China
Ying Chen
Chongqing Engineering Research Center of Goose Genetic Improvement, Institute of Poultry Science, Chongqing Academy of Animal Science, Chongqing 402460, China
Guangliang Gao
Chongqing Engineering Research Center of Goose Genetic Improvement, Institute of Poultry Science, Chongqing Academy of Animal Science, Chongqing 402460, China
Qigui Wang
Chongqing Engineering Research Center of Goose Genetic Improvement, Institute of Poultry Science, Chongqing Academy of Animal Science, Chongqing 402460, China
To explore the impact of epigenetic modifications on egg-laying traits in geese, we employed genome-wide bisulfite sequencing (WGBS) to analyze DNA methylation patterns in pituitary tissues of high-(HYP) and low-yield (LYP) Sichuan White geese. We achieved high-quality sequencing data (mean 19.09 Gb raw reads, 15.49 Gb clean reads, 79.1% unique mapping rate) with a bisulfite conversion efficiency of 99.88%. Comparative analysis revealed 2394 differentially methylated regions (DMRs) and 422 differentially methylated genes (DMGs) between HYP and LYP groups. We identified five key differentially methylated candidate genes (BMPER, INHA, NMBR, NK3R, and DSG2) linked to egg-laying traits in Sichuan White geese. Integrated GO and KEGG enrichment analysis conducted to explore the role of regulatory networks of epigenetic modification on egg-laying traits in Sichuan White geese identified multiple metabolic pathways associated with egg-laying traits (promoting egg transport, ovulation, and yolk protein synthesis and secretion), thus providing a basis for subsequent functional verification.