In Vitro Investigation of Therapeutic and Anti-Coagulant Properties of Allium Sativum L. On Human Plasma

Abstract

Cardio vascular illnesses are increasing worldwide and among these illnesses Thromboembolic disorders are very prevalent. The anticoagulant properties of many herbs have been reported. Garlic is a common herbal supplement. The current study was conducted to compare the in vitro anti-coagulant activity of different solvent-extracted fractions on human blood samples. For this purpose, rectified spirit and reverse osmosis (RO water), were used as solvents. Multiple extracting approaches were applied to prepare different extract fractions of Allium sativum, both in ethanolic and aqueous extracts by maceration, decoction, and soxhlet extraction methods. The concentration of each extract fraction was subjected to a primarily anti-coagulant screening method in human blood sample in-vitro by calculating their prothrombin time of coagulation. . The anti-coagulant activity of the extracts was determined by measuring the changes in prothrombin time with a null hypothesis value of p< 0.05. The results indicated that all garlic extract fractions had significant anti-coagulant potential. However, at 5 ppm concentration, soxhlet extraction extract had the maximum anti-coagulant potential. Moreover, Garlic's aqueous extract also showed a significant anti-coagulant effect on human plasma. This observation agrees with the obtained data that the soxhlet extracted sample of Garlic showed the highest activity of platelet aggregation inhibition. Both ethanolic and aqueous extracts of A. sativum showed significant anti-coagulation properties as compared to positive controlled EDTA and Double oxalate as a synthetic anti-coagulants in our study. Keywords: Allium Sativum L., anti-Coagulant, human plasma, prothrombin time, rectified sprit Copy Right (c) The authors

Keywords

• Allium sativum L.
• Anti-coagulant
• Humane Plasma
• Prothrombin Time
• Rectified Spirt