In Vitro Assessment of the Impact of Industrial Processes on the Gastrointestinal Digestion of Milk Protein Matrices Using the INFOGEST Protocol
Nathalie Atallah,
Barbara Deracinois,
Audrey Boulier,
Alain Baniel,
Delphine Jouan-Rimbaud Bouveresse,
Rozenn Ravallec,
Christophe Flahaut,
Benoit Cudennec
Affiliations
Nathalie Atallah
UMR Transfrontalière BioEcoAgro N° 1158, University Lille, INRAE, University Liège, UPJV, YNCREA, University Artois, University Littoral Côte d’Opale, ICV—Institut Charles Viollette, F-59000 Lille, France
Barbara Deracinois
UMR Transfrontalière BioEcoAgro N° 1158, University Lille, INRAE, University Liège, UPJV, YNCREA, University Artois, University Littoral Côte d’Opale, ICV—Institut Charles Viollette, F-59000 Lille, France
Audrey Boulier
Ingredia S.A. 51 Av. Lobbedez—CS 60946, 62033 Arras CEDEX, France
Alain Baniel
Ingredia S.A. 51 Av. Lobbedez—CS 60946, 62033 Arras CEDEX, France
Delphine Jouan-Rimbaud Bouveresse
AgroParisTech, Université Paris-Saclay, INRAE, UMR PNCA, 75005 Paris, France
Rozenn Ravallec
UMR Transfrontalière BioEcoAgro N° 1158, University Lille, INRAE, University Liège, UPJV, YNCREA, University Artois, University Littoral Côte d’Opale, ICV—Institut Charles Viollette, F-59000 Lille, France
Christophe Flahaut
UMR Transfrontalière BioEcoAgro N° 1158, University Lille, INRAE, University Liège, UPJV, YNCREA, University Artois, University Littoral Côte d’Opale, ICV—Institut Charles Viollette, F-59000 Lille, France
Benoit Cudennec
UMR Transfrontalière BioEcoAgro N° 1158, University Lille, INRAE, University Liège, UPJV, YNCREA, University Artois, University Littoral Côte d’Opale, ICV—Institut Charles Viollette, F-59000 Lille, France
The goal of this study was to determine the impact of industrial processes on the digestion of six milk protein matrices using the harmonized INFOGEST in vitro static digestion protocol. First, this method was optimized to simple protein matrices using sodium dodecyl sulfate-polyacrylamide gel electrophoresis (SDS-PAGE) and size exclusion chromatography (SEC) to compare the intestinal protein hydrolysis obtained with increasing quantities of pancreatin. Similar results were achieved with the originally required pancreatin amount (trypsin activity of 100 U.mL−1) and with a quantity of pancreatin equivalent to a trypsin activity of 27.3 U.mL−1, which was thus used to perform the in vitro digestion of the milk matrices. Molecular weight profiles, peptide heterogeneity from LC-MS/MS data, calcium, free amino acid, and peptide concentrations were determined in the gastric and intestinal phases to compare the milk protein digests. Results showed that the industrial process affected not only the protein distribution of the matrices but also most likely the protein structures. Indeed, differences arose in terms of peptide populations generated when the caseins were reticulated or when their calcium concentrations were reduced.
