Application of Essential Oils to Control the Biodeteriogenic Microorganisms in Archives and Libraries

Abstract

Microbial contamination control in indoor environments, such as libraries and archives, represents a challenge. Essential oils (EOs), well-known for their antimicrobial properties, have been applied in pharmaceutical and food industry from many years. In the present study, Thymus vulgaris and Origanum vulgare EO antimicrobial efficacy on paper-born microorganisms, Staphylococcus epidermidis, Rhodotorula mucilaginosa and Alternaria alternata, was investigated to protect water-damaged paper documents and to control indoor air quality for operator’s health safety. T. vulgaris EO was the most effective: Minimum Inhibitory Concentration (MIC) values obtained for S. epidermidis and R. mucilaginosa, with a broth macro-dilution method, were 7.5 µg/mL and 5.63 µg/mL, respectively. T. vulgaris EO (0.75% v/v), nebulized immediately after the inoculation on agar plates or paper sheets, showed a high inhibition effect against the three biodeteriogenic microorganisms, also when lyophilized on paper sheets; in this last case, the EO has a higher efficacy when applied immediately after the freeze drying. Regarding the EO effect against A. alternata, the inhibition percentage of the mycelial growth, MGI, (81.4%), observed for nonsporulated mycelium, was higher than that for the sporulated one (51.4%). Finally, T. vulgaris EO (0.75% v/v) was effectively applied on a real contaminated book cover by means of EO impregnated contact sheets. Obtained results demonstrated that tested EOs were able to delay or completely inhibit paper-born microorganism growth for both flood-independent or -dependent contamination.

Keywords

• <i>Thymus vulgaris</i> essential oil
• lyophilization
• paper-flooding
• paper-born microorganisms
• <i>A. alternata</i>