Thin-layer chromatography of tissue lipids without extraction

Abstract

Frozen tissue sections were applied directly to silica gel plates, and the lipids were separated by developing the plates in different solvent systems. Quantitation of the lipid classes was achieved by direct transmission densitometry after the plates were treated with a chromic acid-sulfuric acid spray.

Keywords

• histchromatography
• brain lipids
• adipose tissue
• gangliosides
• cholesterol
• phospholipids