Laser-induced autofluorescence for assessment of methabolism and hemodynamic characteristics of the brain

Abstract

The paper contains results of the investigation of the laser-inducedfluorescence detection method for the assessment ofbrain metabolism in situ through the dura mater. Models ofanoxia and acute brain ischemia were used for the evaluation ofreliability of the method utilizing registration of reduced tissuepyridine nucleotides fluorescence, as well as for the assessmentof the viability index, based on the conversion of oxy- anddeoxyhemoglobin. Some pathobiochemical mechanisms ofalterations in the pool ofpyridine nucleotides in anoxia and ischemia were analyzed.

Keywords

• brain
• autofluorescence
• pyridine nucleotides