Plant Growth-Promoting Bacteria Influence Microbial Community Composition and Metabolic Function to Enhance the Efficiency of <i>Hybrid pennisetum</i> Remediation in Cadmium-Contaminated Soil

Zhao-Jin Chen; Meng-Lu Li; Shan-Shan Gao; Yu-Bo Sun; Hui Han; Bai-Lian Li; Yu-Ying Li

doi:10.3390/microorganisms12050870

Microorganisms (Apr 2024)

Plant Growth-Promoting Bacteria Influence Microbial Community Composition and Metabolic Function to Enhance the Efficiency of <i>Hybrid pennisetum</i> Remediation in Cadmium-Contaminated Soil

Zhao-Jin Chen,
Meng-Lu Li,
Shan-Shan Gao,
Yu-Bo Sun,
Hui Han,
Bai-Lian Li,
Yu-Ying Li

Affiliations

Zhao-Jin Chen: Overseas Expertise Introduction Center for Discipline Innovation of Watershed Ecological Security in the Water Source Area of the Mid-line Project of South-to-North Water Diversion, College of Water Resource and Environment Engineering, Nanyang Normal University, Nanyang 473061, China
Meng-Lu Li: Overseas Expertise Introduction Center for Discipline Innovation of Watershed Ecological Security in the Water Source Area of the Mid-line Project of South-to-North Water Diversion, College of Water Resource and Environment Engineering, Nanyang Normal University, Nanyang 473061, China
Shan-Shan Gao: Overseas Expertise Introduction Center for Discipline Innovation of Watershed Ecological Security in the Water Source Area of the Mid-line Project of South-to-North Water Diversion, College of Water Resource and Environment Engineering, Nanyang Normal University, Nanyang 473061, China
Yu-Bo Sun: Overseas Expertise Introduction Center for Discipline Innovation of Watershed Ecological Security in the Water Source Area of the Mid-line Project of South-to-North Water Diversion, College of Water Resource and Environment Engineering, Nanyang Normal University, Nanyang 473061, China
Hui Han: Overseas Expertise Introduction Center for Discipline Innovation of Watershed Ecological Security in the Water Source Area of the Mid-line Project of South-to-North Water Diversion, College of Water Resource and Environment Engineering, Nanyang Normal University, Nanyang 473061, China
Bai-Lian Li: Overseas Expertise Introduction Center for Discipline Innovation of Watershed Ecological Security in the Water Source Area of the Mid-line Project of South-to-North Water Diversion, College of Water Resource and Environment Engineering, Nanyang Normal University, Nanyang 473061, China
Yu-Ying Li: Overseas Expertise Introduction Center for Discipline Innovation of Watershed Ecological Security in the Water Source Area of the Mid-line Project of South-to-North Water Diversion, College of Water Resource and Environment Engineering, Nanyang Normal University, Nanyang 473061, China

DOI: https://doi.org/10.3390/microorganisms12050870
Journal volume & issue: Vol. 12, no. 5
p. 870

Abstract

Read online

The green and efficient remediation of soil cadmium (Cd) is an urgent task, and plant-microbial joint remediation has become a research hotspot due to its advantages. High-throughput sequencing and metabolomics have technical advantages in analyzing the microbiological mechanism of plant growth-promoting bacteria in improving phytoremediation of soil heavy metal pollution. In this experiment, a pot trial was conducted to investigate the effects of inoculating the plant growth-promoting bacterium Enterobacter sp. VY on the growth and Cd remediation efficiency of the energy plant Hybrid pennisetum. The test strain VY-1 was analyzed using high-throughput sequencing and metabolomics to assess its effects on microbial community composition and metabolic function. The results demonstrated that Enterobacter sp. VY-1 effectively mitigated Cd stress on Hybrid pennisetum, resulting in increased plant biomass, Cd accumulation, and translocation factor, thereby enhancing phytoremediation efficiency. Analysis of soil physical-chemical properties revealed that strain VY-1 could increase soil total nitrogen, total phosphorus, available phosphorus, and available potassium content. Principal coordinate analysis (PCoA) indicated that strain VY-1 significantly influenced bacterial community composition, with Proteobacteria, Firmicutes, Chloroflexi, among others, being the main differential taxa. Redundancy analysis (RDA) revealed that available phosphorus, available potassium, and pH were the primary factors affecting bacterial communities. Partial Least Squares Discriminant Analysis (PLS-DA) demonstrated that strain VY-1 modulated the metabolite profile of Hybrid pennisetum rhizosphere soil, with 27 differential metabolites showing significant differences, including 19 up-regulated and eight down-regulated expressions. These differentially expressed metabolites were primarily involved in metabolism and environmental information processing, encompassing pathways such as glutamine and glutamate metabolism, α-linolenic acid metabolism, pyrimidine metabolism, and purine metabolism. This study utilized 16S rRNA high-throughput sequencing and metabolomics technology to investigate the impact of the plant growth-promoting bacterium Enterobacter sp. VY-1 on the growth and Cd enrichment of Hybrid pennisetum, providing insights into the regulatory role of plant growth-promoting bacteria in microbial community structure and metabolic function, thereby improving the microbiological mechanisms of phytoremediation.

Published in Microorganisms

ISSN: 2076-2607 (Online)
Publisher: MDPI AG
Country of publisher: Switzerland
LCC subjects: Science: Biology (General)
Website: https://www.mdpi.com/journal/microorganisms

About the journal

Abstract

Keywords