Rapid detection of human mastadenovirus species B by recombinase polymerase amplification assay

Tao Wu; Haizhen Wu; Kangchen Zhao; Chaoyou Hu; Yiyue Ge; Xiaojuan Zhu; Xingchen Zhang; Minghao Zhou; Fengcai Zhu; Lunbiao Cui

doi:10.1186/s12866-018-1365-7

BMC Microbiology (Jan 2019)

Rapid detection of human mastadenovirus species B by recombinase polymerase amplification assay

Tao Wu,
Haizhen Wu,
Kangchen Zhao,
Chaoyou Hu,
Yiyue Ge,
Xiaojuan Zhu,
Xingchen Zhang,
Minghao Zhou,
Fengcai Zhu,
Lunbiao Cui

Affiliations

Tao Wu: Institute of Pathogenic Microbiology, Key Laboratories of Enteric Pathogenic Microbiology (Ministry of Health), Jiangsu Provincial Center for Disease Control and Prevention
Haizhen Wu: Kunshan Municipal Center for Disease Control and Prevention
Kangchen Zhao: Institute of Pathogenic Microbiology, Key Laboratories of Enteric Pathogenic Microbiology (Ministry of Health), Jiangsu Provincial Center for Disease Control and Prevention
Chaoyou Hu: Kunshan Municipal Center for Disease Control and Prevention
Yiyue Ge: Institute of Pathogenic Microbiology, Key Laboratories of Enteric Pathogenic Microbiology (Ministry of Health), Jiangsu Provincial Center for Disease Control and Prevention
Xiaojuan Zhu: Institute of Pathogenic Microbiology, Key Laboratories of Enteric Pathogenic Microbiology (Ministry of Health), Jiangsu Provincial Center for Disease Control and Prevention
Xingchen Zhang: Kunshan Municipal Center for Disease Control and Prevention
Minghao Zhou: Institute of Pathogenic Microbiology, Key Laboratories of Enteric Pathogenic Microbiology (Ministry of Health), Jiangsu Provincial Center for Disease Control and Prevention
Fengcai Zhu: Institute of Pathogenic Microbiology, Key Laboratories of Enteric Pathogenic Microbiology (Ministry of Health), Jiangsu Provincial Center for Disease Control and Prevention
Lunbiao Cui: Institute of Pathogenic Microbiology, Key Laboratories of Enteric Pathogenic Microbiology (Ministry of Health), Jiangsu Provincial Center for Disease Control and Prevention

DOI: https://doi.org/10.1186/s12866-018-1365-7
Journal volume & issue: Vol. 19, no. 1
pp. 1 – 7

Abstract

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Abstract Background As an important component of the causative agent of respiratory tract infections, enteric and eye infections, Human mastadenoviruses (HAdVs) species B spread easily in the crowd. In this study, we developed a recombinase polymerase amplification (RPA) assay for rapidly detecting HAdVs species B which was comprised of two different formats (real-time and lateral-flow device). Results This assay was confirmed to be able to detect 5 different HAdVs species B subtypes (HAdV-B3, HAdV-B7, HAdV-B11, HAdV-B14 and HAdV-B55) without cross-reactions with other subtypes and other respiratory tract pathogens. This RPA assay has not only highly sensitivity with low detection limit of 50 copies per reaction but also short reaction time (< 15 min per detection). Furthermore, the real-time RPA assay has excellent correlation with real-time PCR assay for detection of HAdVs species B presented in clinical samples. Conclusions Thus, the RPA assay developed in this study provides an effective and portable approach for the rapid detection of HAdVs species B.

Published in BMC Microbiology

ISSN: 1471-2180 (Online)
Publisher: BMC
Country of publisher: United Kingdom
LCC subjects: Science: Microbiology
Website: http://www.biomedcentral.com/bmcmicrobiol/

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