An Electrochemical DNA Biosensor for Carcinogenicity of Anticancer Compounds Based on Competition between Methylene Blue and Oligonucleotides
Nor Diyana Md. Sani,
Eda Yuhana Ariffin,
Wong Sheryn,
Mohd Asyraf Shamsuddin,
Lee Yook Heng,
Jalifah Latip,
Siti Aishah Hasbullah,
Nurul Izzaty Hassan
Affiliations
Nor Diyana Md. Sani
Sanichem Resources Sdn Bhd, No 7 & 7a, Jalan Timur 6/1a, Mercato Enstek, Bandar Enstek NSN 71060, Malaysia
Eda Yuhana Ariffin
Center for Advanced Materials & Renewable Resources, Faculty of Science & Technology, Universiti Kebangsaan Malaysia, Bandar Baru Bangi 43600, Selangor Darul Ehsan, Malaysia
Wong Sheryn
Center for Advanced Materials & Renewable Resources, Faculty of Science & Technology, Universiti Kebangsaan Malaysia, Bandar Baru Bangi 43600, Selangor Darul Ehsan, Malaysia
Mohd Asyraf Shamsuddin
Center for Advanced Materials & Renewable Resources, Faculty of Science & Technology, Universiti Kebangsaan Malaysia, Bandar Baru Bangi 43600, Selangor Darul Ehsan, Malaysia
Lee Yook Heng
Center for Advanced Materials & Renewable Resources, Faculty of Science & Technology, Universiti Kebangsaan Malaysia, Bandar Baru Bangi 43600, Selangor Darul Ehsan, Malaysia
Jalifah Latip
Center for Advanced Materials & Renewable Resources, Faculty of Science & Technology, Universiti Kebangsaan Malaysia, Bandar Baru Bangi 43600, Selangor Darul Ehsan, Malaysia
Siti Aishah Hasbullah
Center for Advanced Materials & Renewable Resources, Faculty of Science & Technology, Universiti Kebangsaan Malaysia, Bandar Baru Bangi 43600, Selangor Darul Ehsan, Malaysia
Nurul Izzaty Hassan
Center for Advanced Materials & Renewable Resources, Faculty of Science & Technology, Universiti Kebangsaan Malaysia, Bandar Baru Bangi 43600, Selangor Darul Ehsan, Malaysia
A toxicity electrochemical DNA biosensor has been constructed for the detection of carcinogens using 24 base guanine DNA rich single stranded DNA, and methylene blue (MB) as the electroactive indicator. This amine terminated ssDNA was immobilized onto silica nanospheres and deposited on gold nanoparticle modified carbon-paste screen printed electrodes (SPEs). The modified SPE was initially exposed to a carcinogen, followed by immersion in methylene blue for an optimized duration. The biosensor response was measured using differential pulse voltammetry. The performance of the biosensor was identified on several anti-cancer compounds. The toxicity DNA biosensor demonstrated a linear response range to the cadmium chloride from 0.0005 ppm to 0.01 ppm (R2 = 0.928) with a limit of detection at 0.0004 ppm. The biosensor also exhibited its versatility to screen the carcinogenicity of potential anti-cancer compounds.
